Stable transformation of maize after gene transfer by electroporation

Michael E. Fromm, Loverine P. Taylor, Virginia Walbot

Research output: Contribution to journalArticle

380 Citations (Scopus)

Abstract

The graminaceous monocots, including the economically important cereals, seem to be refractory to infection by Agrobacterium tumefaciens1, a natural gene transfer system that has been successfully exploited for transferring foreign genes into higher plants2-4. Therefore, direct transfer techniques that are potentially applicable to all plant species have been developed using a few dicot5-8 and monocot8-10 species as model systems. One of these techniques, electroporation, uses electrical pulses of high field strength to permeabilize cell membranes 11 reversibly so as to facilitate the transfer of DNA into cells 8,12,13. Electroporation-mediated gene transfer has resulted in stably transformed animal cells12,13 and transient gene expression in monocot and dicot plant cells8. Here we report that electroporation-mediated DNA transfer of a chimaeric gene encoding neomycin phosphotransferase results in stably transformed maize cells that are resistant to kanamycin.

Original languageEnglish (US)
Pages (from-to)791-793
Number of pages3
JournalNature
Volume319
Issue number6056
DOIs
StatePublished - Dec 1 1986

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Electroporation
Zea mays
Genes
Kanamycin Kinase
Agrobacterium
Kanamycin
DNA
Cell Membrane
Gene Expression
Infection

ASJC Scopus subject areas

  • General

Cite this

Fromm, M. E., Taylor, L. P., & Walbot, V. (1986). Stable transformation of maize after gene transfer by electroporation. Nature, 319(6056), 791-793. https://doi.org/10.1038/319791a0

Stable transformation of maize after gene transfer by electroporation. / Fromm, Michael E.; Taylor, Loverine P.; Walbot, Virginia.

In: Nature, Vol. 319, No. 6056, 01.12.1986, p. 791-793.

Research output: Contribution to journalArticle

Fromm, ME, Taylor, LP & Walbot, V 1986, 'Stable transformation of maize after gene transfer by electroporation', Nature, vol. 319, no. 6056, pp. 791-793. https://doi.org/10.1038/319791a0
Fromm, Michael E. ; Taylor, Loverine P. ; Walbot, Virginia. / Stable transformation of maize after gene transfer by electroporation. In: Nature. 1986 ; Vol. 319, No. 6056. pp. 791-793.
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