Src directly phosphorylates Bif-1 and prevents its interaction with Bax and the initiation of anoikis

Hirohito Yamaguchi, Nicholas T Woods, Jay F. Dorsey, Yoshinori Takahashi, Nicole R. Gjertsen, Timothy Yeatman, Jie Wu, Hong Gang Wang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Bif-1 interacts with Bax and enhances its conformational rearrangement, resulting in apoptosis. However, the molecular mechanism governing the interaction between Bif-1 and Bax is poorly defined. Here we provide evidence that Bif-1 is phosphorylated, an event that can be repressed by apoptotic stimuli. The protein kinase c-Src binds to and directly phosphorylates Bif-1 on tyrosine 80. Moreover, Src phosphorylation of Bif-1 suppresses the interaction between Bif-1 and Bax, resulting in the inhibition of Bax activation during anoikis. Together, these results suggest that phosphorylation of Bif-1 impairs its binding to Bax and represses apoptosis, providing another mechanism by which Src oncogenic signaling can prevent cell death.

Original languageEnglish (US)
Pages (from-to)19112-19118
Number of pages7
JournalJournal of Biological Chemistry
Volume283
Issue number27
DOIs
StatePublished - Jul 4 2008

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Anoikis
Phosphorylation
Apoptosis
Cell death
Protein Kinases
Tyrosine
Cell Death
Chemical activation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yamaguchi, H., Woods, N. T., Dorsey, J. F., Takahashi, Y., Gjertsen, N. R., Yeatman, T., ... Wang, H. G. (2008). Src directly phosphorylates Bif-1 and prevents its interaction with Bax and the initiation of anoikis. Journal of Biological Chemistry, 283(27), 19112-19118. https://doi.org/10.1074/jbc.M709882200

Src directly phosphorylates Bif-1 and prevents its interaction with Bax and the initiation of anoikis. / Yamaguchi, Hirohito; Woods, Nicholas T; Dorsey, Jay F.; Takahashi, Yoshinori; Gjertsen, Nicole R.; Yeatman, Timothy; Wu, Jie; Wang, Hong Gang.

In: Journal of Biological Chemistry, Vol. 283, No. 27, 04.07.2008, p. 19112-19118.

Research output: Contribution to journalArticle

Yamaguchi, H, Woods, NT, Dorsey, JF, Takahashi, Y, Gjertsen, NR, Yeatman, T, Wu, J & Wang, HG 2008, 'Src directly phosphorylates Bif-1 and prevents its interaction with Bax and the initiation of anoikis', Journal of Biological Chemistry, vol. 283, no. 27, pp. 19112-19118. https://doi.org/10.1074/jbc.M709882200
Yamaguchi, Hirohito ; Woods, Nicholas T ; Dorsey, Jay F. ; Takahashi, Yoshinori ; Gjertsen, Nicole R. ; Yeatman, Timothy ; Wu, Jie ; Wang, Hong Gang. / Src directly phosphorylates Bif-1 and prevents its interaction with Bax and the initiation of anoikis. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 27. pp. 19112-19118.
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