Spotted leaf11, a negative regulator of plant cell death and defense, encodes a U-box/armadillo repeat protein endowed with E3 ubiquitin ligase activity

Li Rong Zeng, Shaohong Qu, Alicia Bordeos, Chengwei Yang, Marietta Baraoidan, Hongyan Yan, Qi Xie, Hie Nahm Baek, Hei Leung, Guo Liang Wang

Research output: Contribution to journalArticle

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Abstract

The rice (Oryza sativa) spotted leaf11 (spl11) mutant was identified from an ethyl methanesulfonate-mutagenized indica cultivar IR68 population and was previously shown to display a spontaneous cell death phenotype and enhanced resistance to rice fungal and bacterial pathogens. Here, we have isolated Spl11 via a map-based cloning strategy. The isolation of the Spl11 gene was facilitated by the identification of three additional spl11 alleles from an IR64 mutant collection. The predicted SPL11 protein contains both a U-box domain and an armadillo (ARM) repeat domain, which were demonstrated in yeast and mammalian systems to be involved in ubiquitination and protein-protein interactions, respectively. Amino acid sequence comparison indicated that the similarity between SPL11 and other plant U-box-ARM proteins is mostly restricted to the U-box and ARM repeat regions. A single base substitution was detected in spl11, which results in a premature stop codon in the SPL11 protein. Expression analysis indicated that Spl11 is induced in both incompatible and compatible rice-blast interactions. In vitro ubiquitination assay indicated that the SPL11 protein possesses E3 ubiquitin ligase activity that is dependent on an intact U-box domain, suggesting a role of the ubiquitination system in the control of plant cell death and defense.

Original languageEnglish (US)
Pages (from-to)2795-2808
Number of pages14
JournalPlant Cell
Volume16
Issue number10
DOIs
StatePublished - Oct 1 2004

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Armadillo Domain Proteins
Dasypodidae
ubiquitin-protein ligase
Ubiquitin-Protein Ligases
Plant Cells
Cell death
cell death
Ubiquitination
Cell Death
Armadillos
rice
ethyl methanesulfonate
mutants
Proteins
blast disease
proteins
stop codon
protein-protein interactions
Ethyl Methanesulfonate
Nonsense Codon

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Spotted leaf11, a negative regulator of plant cell death and defense, encodes a U-box/armadillo repeat protein endowed with E3 ubiquitin ligase activity. / Zeng, Li Rong; Qu, Shaohong; Bordeos, Alicia; Yang, Chengwei; Baraoidan, Marietta; Yan, Hongyan; Xie, Qi; Baek, Hie Nahm; Leung, Hei; Wang, Guo Liang.

In: Plant Cell, Vol. 16, No. 10, 01.10.2004, p. 2795-2808.

Research output: Contribution to journalArticle

Zeng, Li Rong ; Qu, Shaohong ; Bordeos, Alicia ; Yang, Chengwei ; Baraoidan, Marietta ; Yan, Hongyan ; Xie, Qi ; Baek, Hie Nahm ; Leung, Hei ; Wang, Guo Liang. / Spotted leaf11, a negative regulator of plant cell death and defense, encodes a U-box/armadillo repeat protein endowed with E3 ubiquitin ligase activity. In: Plant Cell. 2004 ; Vol. 16, No. 10. pp. 2795-2808.
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AU - Yan, Hongyan

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