Spleen but not tumor infiltration by dendritic and T cells is increased by intravenous adenovirus-Flt3 ligand injection

Joyce C Solheim, A. J. Reber, A. E. Ashour, S. Robinson, M. Futakuchi, S. G. Kurz, K. Hood, R. R. Fields, L. R. Shafer, D. Cornell, S. Sutjipto, S. Zurawski, D. M. LaFace, Rakesh K Singh, James E Talmadge

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Dendritic cell (DC) expansion is regulated by the hematopoietic growth factor fms-like tyrosine kinase 3 ligand (Flt3L). DCs are critical to the control of tumor growth and metastasis, and there is a positive correlation between intratumoral DC infiltration and clinical outcome. In this report, we first demonstrate that single intravenous (i.v.) injections of adenovirus (Adv)-Flt3L significantly increased splenic dendritic, B, T and natural killer (NK) cell numbers in both normal and mammary tumor-bearing mice. In contrast, the numbers of DCs and T cells infiltrating the tumors were not increased. Consistent with the minimal effect on immune cell infiltration, i.v. Adv-Flt3L injections had no therapeutic activity against orthotopic mammary tumors. In addition, we noted tumor and Adv-Flt3L expansion of Gr1+CD11b + immature myeloid suppressor cells (IMSCs), which may inhibit the therapeutic efficacy of Adv-Flt3L-expanded DCs.

Original languageEnglish (US)
Pages (from-to)364-371
Number of pages8
JournalCancer Gene Therapy
Volume14
Issue number4
DOIs
StatePublished - Apr 1 2007

Fingerprint

Adenoviridae
Dendritic Cells
Spleen
T-Lymphocytes
Injections
Neoplasms
Breast Neoplasms
Myeloid Cells
Intravenous Injections
Intercellular Signaling Peptides and Proteins
Cell Count
flt3 ligand protein
Neoplasm Metastasis
Therapeutics
Growth

Keywords

  • Breast cancer
  • Cytokine
  • Dendritic cell
  • Flt3L
  • Mammary tumor

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cancer Research

Cite this

Spleen but not tumor infiltration by dendritic and T cells is increased by intravenous adenovirus-Flt3 ligand injection. / Solheim, Joyce C; Reber, A. J.; Ashour, A. E.; Robinson, S.; Futakuchi, M.; Kurz, S. G.; Hood, K.; Fields, R. R.; Shafer, L. R.; Cornell, D.; Sutjipto, S.; Zurawski, S.; LaFace, D. M.; Singh, Rakesh K; Talmadge, James E.

In: Cancer Gene Therapy, Vol. 14, No. 4, 01.04.2007, p. 364-371.

Research output: Contribution to journalArticle

Solheim, JC, Reber, AJ, Ashour, AE, Robinson, S, Futakuchi, M, Kurz, SG, Hood, K, Fields, RR, Shafer, LR, Cornell, D, Sutjipto, S, Zurawski, S, LaFace, DM, Singh, RK & Talmadge, JE 2007, 'Spleen but not tumor infiltration by dendritic and T cells is increased by intravenous adenovirus-Flt3 ligand injection', Cancer Gene Therapy, vol. 14, no. 4, pp. 364-371. https://doi.org/10.1038/sj.cgt.7701018
Solheim, Joyce C ; Reber, A. J. ; Ashour, A. E. ; Robinson, S. ; Futakuchi, M. ; Kurz, S. G. ; Hood, K. ; Fields, R. R. ; Shafer, L. R. ; Cornell, D. ; Sutjipto, S. ; Zurawski, S. ; LaFace, D. M. ; Singh, Rakesh K ; Talmadge, James E. / Spleen but not tumor infiltration by dendritic and T cells is increased by intravenous adenovirus-Flt3 ligand injection. In: Cancer Gene Therapy. 2007 ; Vol. 14, No. 4. pp. 364-371.
@article{83369c1db1864eeb96ca3933079111ab,
title = "Spleen but not tumor infiltration by dendritic and T cells is increased by intravenous adenovirus-Flt3 ligand injection",
abstract = "Dendritic cell (DC) expansion is regulated by the hematopoietic growth factor fms-like tyrosine kinase 3 ligand (Flt3L). DCs are critical to the control of tumor growth and metastasis, and there is a positive correlation between intratumoral DC infiltration and clinical outcome. In this report, we first demonstrate that single intravenous (i.v.) injections of adenovirus (Adv)-Flt3L significantly increased splenic dendritic, B, T and natural killer (NK) cell numbers in both normal and mammary tumor-bearing mice. In contrast, the numbers of DCs and T cells infiltrating the tumors were not increased. Consistent with the minimal effect on immune cell infiltration, i.v. Adv-Flt3L injections had no therapeutic activity against orthotopic mammary tumors. In addition, we noted tumor and Adv-Flt3L expansion of Gr1+CD11b + immature myeloid suppressor cells (IMSCs), which may inhibit the therapeutic efficacy of Adv-Flt3L-expanded DCs.",
keywords = "Breast cancer, Cytokine, Dendritic cell, Flt3L, Mammary tumor",
author = "Solheim, {Joyce C} and Reber, {A. J.} and Ashour, {A. E.} and S. Robinson and M. Futakuchi and Kurz, {S. G.} and K. Hood and Fields, {R. R.} and Shafer, {L. R.} and D. Cornell and S. Sutjipto and S. Zurawski and LaFace, {D. M.} and Singh, {Rakesh K} and Talmadge, {James E}",
year = "2007",
month = "4",
day = "1",
doi = "10.1038/sj.cgt.7701018",
language = "English (US)",
volume = "14",
pages = "364--371",
journal = "Cancer Gene Therapy",
issn = "0929-1903",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Spleen but not tumor infiltration by dendritic and T cells is increased by intravenous adenovirus-Flt3 ligand injection

AU - Solheim, Joyce C

AU - Reber, A. J.

AU - Ashour, A. E.

AU - Robinson, S.

AU - Futakuchi, M.

AU - Kurz, S. G.

AU - Hood, K.

AU - Fields, R. R.

AU - Shafer, L. R.

AU - Cornell, D.

AU - Sutjipto, S.

AU - Zurawski, S.

AU - LaFace, D. M.

AU - Singh, Rakesh K

AU - Talmadge, James E

PY - 2007/4/1

Y1 - 2007/4/1

N2 - Dendritic cell (DC) expansion is regulated by the hematopoietic growth factor fms-like tyrosine kinase 3 ligand (Flt3L). DCs are critical to the control of tumor growth and metastasis, and there is a positive correlation between intratumoral DC infiltration and clinical outcome. In this report, we first demonstrate that single intravenous (i.v.) injections of adenovirus (Adv)-Flt3L significantly increased splenic dendritic, B, T and natural killer (NK) cell numbers in both normal and mammary tumor-bearing mice. In contrast, the numbers of DCs and T cells infiltrating the tumors were not increased. Consistent with the minimal effect on immune cell infiltration, i.v. Adv-Flt3L injections had no therapeutic activity against orthotopic mammary tumors. In addition, we noted tumor and Adv-Flt3L expansion of Gr1+CD11b + immature myeloid suppressor cells (IMSCs), which may inhibit the therapeutic efficacy of Adv-Flt3L-expanded DCs.

AB - Dendritic cell (DC) expansion is regulated by the hematopoietic growth factor fms-like tyrosine kinase 3 ligand (Flt3L). DCs are critical to the control of tumor growth and metastasis, and there is a positive correlation between intratumoral DC infiltration and clinical outcome. In this report, we first demonstrate that single intravenous (i.v.) injections of adenovirus (Adv)-Flt3L significantly increased splenic dendritic, B, T and natural killer (NK) cell numbers in both normal and mammary tumor-bearing mice. In contrast, the numbers of DCs and T cells infiltrating the tumors were not increased. Consistent with the minimal effect on immune cell infiltration, i.v. Adv-Flt3L injections had no therapeutic activity against orthotopic mammary tumors. In addition, we noted tumor and Adv-Flt3L expansion of Gr1+CD11b + immature myeloid suppressor cells (IMSCs), which may inhibit the therapeutic efficacy of Adv-Flt3L-expanded DCs.

KW - Breast cancer

KW - Cytokine

KW - Dendritic cell

KW - Flt3L

KW - Mammary tumor

UR - http://www.scopus.com/inward/record.url?scp=33947244084&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947244084&partnerID=8YFLogxK

U2 - 10.1038/sj.cgt.7701018

DO - 10.1038/sj.cgt.7701018

M3 - Article

C2 - 17235356

AN - SCOPUS:33947244084

VL - 14

SP - 364

EP - 371

JO - Cancer Gene Therapy

JF - Cancer Gene Therapy

SN - 0929-1903

IS - 4

ER -