Sphingolipids containing very-long-chain fatty acids define a secretory pathway for specific polar plasma membrane protein targeting in arabidopsis

Jonathan E. Markham, Diana Molino, Lionel Gissot, Yannick Bellec, Kian Hématy, Jessica Marion, Katia Belcram, Jean Christophe Palauqui, Béatrice Satiat-Jeunemaître, Jean Denis Faure

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Abstract

Sphingolipids are a class of structural membrane lipids involved in membrane trafficking and cell polarity. Functional analysis of the ceramide synthase family in Arabidopsis thaliana demonstrates the existence of two activities selective for the length of the acyl chains. Very-long-acyl-chain (C > 18 carbons) but not long-chain sphingolipids are essential for plant development. Reduction of very-long-chain fatty acid sphingolipid levels leads in particular to auxin-dependent inhibition of lateral root emergence that is associated with selective aggregation of the plasma membrane auxin carriers AUX1 and PIN1 in the cytosol. Defective targeting of polar auxin carriers is characterized by specific aggregation of Rab-A2 a- and Rab-A1 e- labeled early endosomes along the secretory pathway. These aggregates correlate with the accumulation of membrane structures and vesicle fragmentation in the cytosol. In conclusion, sphingolipids with very long acyl chains define a trafficking pathway with specific endomembrane compartments and polar auxin transport protein cargoes.

Original languageEnglish (US)
Pages (from-to)2362-2378
Number of pages17
JournalPlant Cell
Volume23
Issue number6
DOIs
StatePublished - Jun 1 2011

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very long chain fatty acids
sphingolipids
Sphingolipids
Indoleacetic Acids
Secretory Pathway
Protein Transport
Arabidopsis
membrane proteins
blood proteins
Blood Proteins
auxins
Membrane Proteins
Fatty Acids
plasma membrane
Cell Membrane
cytosol
Cytosol
Cell Polarity
endosomes
Plant Development

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Sphingolipids containing very-long-chain fatty acids define a secretory pathway for specific polar plasma membrane protein targeting in arabidopsis. / Markham, Jonathan E.; Molino, Diana; Gissot, Lionel; Bellec, Yannick; Hématy, Kian; Marion, Jessica; Belcram, Katia; Palauqui, Jean Christophe; Satiat-Jeunemaître, Béatrice; Faure, Jean Denis.

In: Plant Cell, Vol. 23, No. 6, 01.06.2011, p. 2362-2378.

Research output: Contribution to journalArticle

Markham, JE, Molino, D, Gissot, L, Bellec, Y, Hématy, K, Marion, J, Belcram, K, Palauqui, JC, Satiat-Jeunemaître, B & Faure, JD 2011, 'Sphingolipids containing very-long-chain fatty acids define a secretory pathway for specific polar plasma membrane protein targeting in arabidopsis', Plant Cell, vol. 23, no. 6, pp. 2362-2378. https://doi.org/10.1105/tpc.110.080473
Markham, Jonathan E. ; Molino, Diana ; Gissot, Lionel ; Bellec, Yannick ; Hématy, Kian ; Marion, Jessica ; Belcram, Katia ; Palauqui, Jean Christophe ; Satiat-Jeunemaître, Béatrice ; Faure, Jean Denis. / Sphingolipids containing very-long-chain fatty acids define a secretory pathway for specific polar plasma membrane protein targeting in arabidopsis. In: Plant Cell. 2011 ; Vol. 23, No. 6. pp. 2362-2378.
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