Sphingolipids are required for efficient triacylglycerol loss in conjugated linoleic acid treated adipocytes

Wei Wang, Michael E Fromm

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Conjugated linoleic acid (CLA) reduces adiposity in human and mouse adipocytes. This outcome is achieved through a variety of biological responses including increased energy expenditure and fatty acid oxidation, increased inflammation, repression of fatty acid biosynthesis, attenuated glucose transport, and apoptosis. In the current study, profiling of 261 metabolites was conducted to gain new insights into the biological pathways responding to CLA in 3T3-L1 adipocytes. Sphinganine and sphingosine levels were observed to be highly elevated in CLA treated adipocytes. Exogenous chemicals that increased endogenous ceramide levels decreased lipid levels in adipocytes, and activated AMP-activated protein kinase (AMPK) as well as NF-κB, both of which are typically activated in CLA treated adipocytes. Concurrent inhibition of ceramide de novo biosynthesis and recycling from existing sphingolipid pools attenuated the lipid lowering effect normally associated with responses to CLA, implicating ceramides as an important component of the lipid lowering response in CLA treated adipocytes.

Original languageEnglish (US)
Article numbere0119005
JournalPloS one
Volume10
Issue number4
DOIs
StatePublished - Apr 23 2015

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Conjugated Linoleic Acids
sphingolipids
Sphingolipids
conjugated linoleic acid
adipocytes
Adipocytes
Triglycerides
triacylglycerols
ceramides
Ceramides
Biosynthesis
Lipids
Fatty Acids
biosynthesis
lipemic effect
sphingosine
AMP-activated protein kinase
AMP-Activated Protein Kinases
Sphingosine
beta oxidation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Sphingolipids are required for efficient triacylglycerol loss in conjugated linoleic acid treated adipocytes. / Wang, Wei; Fromm, Michael E.

In: PloS one, Vol. 10, No. 4, e0119005, 23.04.2015.

Research output: Contribution to journalArticle

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