Spectroscopic and computational studies of the ATP:corrinoid adenosyltransferase (CobA) from Salmonella enterica: Insights into the mechanism of adenosylcobalamin biosynthesis

Troy A. Stich, Nicole R. Buan, Jorge C. Escalante-Semerena, Thomas C. Brunold

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

CobA from Salmonella enterica is a member of an enzymatic system responsible for the de novo biosynthesis of adenosylcobalamin (AdoCbl), catalyzing the formation of the essential Co-C bond by transferring the adenosyl group from a molecule of ATP to a transient Co1+corrinoid species generated in the enzyme active site. A particularly fascinating aspect of this reaction is that the flavodoxin in vivo reducing agent that serves as the electron donor to CobA possesses a reduction potential that is considerably more positive than that of the Co2+/1+ couple of the corrinoid substrate. To explore how CobA may overcome this challenge, we have employed electronic absorption, magnetic circular dichroism, and electron paramagnetic resonance (EPR) spectroscopies to probe the interaction between Co3+- and Co2+corrinoids and the enzyme active site. Our data reveal that while Co3+corrinoids interact only weakly with CobA, Co 2+corrinoids undergo partial conversion to a new paramagnetic species that can be obtained in nearly quantitative yield when CobA is preincubated with the co-substrate ATP. This "activated" species is characterized by a distinct set of ligand field transitions in the near-IR spectral region and EPR parameters that are unprecedented for Co2+corrinoids. Analysis of these data on the basis of qualitative spectral correlations and density functional theory computations reveals that this unique Co2+corrinoid species possesses an essentially square-planar Co2+ center that lacks any significant axial bonding interactions. Possible implications of these findings for the mechanism of Co2+ → Co1+ reduction employed by CobA and Co-C bond-forming enzymes in general are explored.

Original languageEnglish (US)
Pages (from-to)8710-8719
Number of pages10
JournalJournal of the American Chemical Society
Volume127
Issue number24
DOIs
StatePublished - Jun 22 2005

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Corrinoids
Salmonella
Salmonella enterica
Adenosinetriphosphate
Biosynthesis
Enzymes
Paramagnetic resonance
Dichroism
Reducing agents
Substrates
Electron Spin Resonance Spectroscopy
Density functional theory
Ligands
Catalytic Domain
Spectroscopy
Adenosine Triphosphate
Flavodoxin
Molecules
Electrons
Reducing Agents

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Spectroscopic and computational studies of the ATP:corrinoid adenosyltransferase (CobA) from Salmonella enterica : Insights into the mechanism of adenosylcobalamin biosynthesis. / Stich, Troy A.; Buan, Nicole R.; Escalante-Semerena, Jorge C.; Brunold, Thomas C.

In: Journal of the American Chemical Society, Vol. 127, No. 24, 22.06.2005, p. 8710-8719.

Research output: Contribution to journalArticle

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abstract = "CobA from Salmonella enterica is a member of an enzymatic system responsible for the de novo biosynthesis of adenosylcobalamin (AdoCbl), catalyzing the formation of the essential Co-C bond by transferring the adenosyl group from a molecule of ATP to a transient Co1+corrinoid species generated in the enzyme active site. A particularly fascinating aspect of this reaction is that the flavodoxin in vivo reducing agent that serves as the electron donor to CobA possesses a reduction potential that is considerably more positive than that of the Co2+/1+ couple of the corrinoid substrate. To explore how CobA may overcome this challenge, we have employed electronic absorption, magnetic circular dichroism, and electron paramagnetic resonance (EPR) spectroscopies to probe the interaction between Co3+- and Co2+corrinoids and the enzyme active site. Our data reveal that while Co3+corrinoids interact only weakly with CobA, Co 2+corrinoids undergo partial conversion to a new paramagnetic species that can be obtained in nearly quantitative yield when CobA is preincubated with the co-substrate ATP. This {"}activated{"} species is characterized by a distinct set of ligand field transitions in the near-IR spectral region and EPR parameters that are unprecedented for Co2+corrinoids. Analysis of these data on the basis of qualitative spectral correlations and density functional theory computations reveals that this unique Co2+corrinoid species possesses an essentially square-planar Co2+ center that lacks any significant axial bonding interactions. Possible implications of these findings for the mechanism of Co2+ → Co1+ reduction employed by CobA and Co-C bond-forming enzymes in general are explored.",
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