Spectroscopic analysis of diversity of arabinoxylan structures in endosperm cell walls of wheat cultivars (Triticum aestivum) in the HEALTHGRAIN diversity collection

Geraldine A. Toole, Gwénaëlle Le Gall, Ian J. Colquhoun, Philip E Johnson, Zoltan Bedö, Luc Saulnier, Peter R. Shewry, E. N.Clare Mills

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Fifty bread wheat (Triticum aestivum L.) cultivars were selected from the HEALTHGRAIN germplasm collection based on variation in their contents of total and water-extractable arabinoxylan. FT-IR spectroscopic mapping of thin transverse sections of grain showed variation in cell wall arabinoxylan composition between the cultivars, from consisting almost entirely of low-substituted arabinoxylan (e.g., T.aestivum Claire) to almost entirely of highly substituted arabinoxylan (e.g., T.aestivum Manital) and a mixture of the two forms (e.g., T.aestivum Hereward). Complementary data were obtained using endoxylanase digestion of flour followed by HP-AEC analysis of the arabinoxylan oligosaccharides. This allowed the selection of six cultivars for more detailed analysis using FT-IR and 1H NMR spectroscopy to determine the proportions of mono-, di-, and unsubstituted xylose residues. The results of the two analyses were consistent, showing that variation in the composition and structure of the endosperm cell wall arabinoxylan is present between bread wheat cultivars. The heterogeneity and spatial distribution of the arabinoxylan in endosperm cell walls may be exploited in wheat processing as it may allow the production of mill streams enriched in various arabinoxylan fractions which have beneficial effects on health.

Original languageEnglish (US)
Pages (from-to)7075-7082
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number13
DOIs
StatePublished - Jul 13 2011

Fingerprint

Endosperm
arabinoxylan
Spectroscopic analysis
Cell Wall
Triticum
endosperm
Triticum aestivum
Cells
cell walls
wheat
cultivars
Bread
endo-1,4-beta-xylanase
Endo-1,4-beta Xylanases
Xylose
Flour
germplasm conservation
Chemical analysis
xylose
Oligosaccharides

Keywords

  • Arabinoxylan
  • FT-IR
  • H NMR
  • HEALTHGRAIN
  • endosperm cell walls
  • wheat

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Spectroscopic analysis of diversity of arabinoxylan structures in endosperm cell walls of wheat cultivars (Triticum aestivum) in the HEALTHGRAIN diversity collection. / Toole, Geraldine A.; Le Gall, Gwénaëlle; Colquhoun, Ian J.; Johnson, Philip E; Bedö, Zoltan; Saulnier, Luc; Shewry, Peter R.; Mills, E. N.Clare.

In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 13, 13.07.2011, p. 7075-7082.

Research output: Contribution to journalArticle

Toole, Geraldine A. ; Le Gall, Gwénaëlle ; Colquhoun, Ian J. ; Johnson, Philip E ; Bedö, Zoltan ; Saulnier, Luc ; Shewry, Peter R. ; Mills, E. N.Clare. / Spectroscopic analysis of diversity of arabinoxylan structures in endosperm cell walls of wheat cultivars (Triticum aestivum) in the HEALTHGRAIN diversity collection. In: Journal of Agricultural and Food Chemistry. 2011 ; Vol. 59, No. 13. pp. 7075-7082.
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