Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae

Margaret I. Boulton, Wallace G. Buchholz, Melanie S. Marks, Peter G. Markham, Jeffrey W. Davies

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Parameters affecting the efficiency of agroinfection of maize streak virus (MSV) in maize have been determined. Monomeric units, cloned at a number of sites in the MSV genome were not infectious but multimeric units containing partial duplications were equally as infectious as complete tandem dimeric clones. Inoculation of tandem dimeric units conjugated into different strains of Agrobacterium showed that both A. tumefaciens and A. rhizogenes were able to transfer DNA to maize and this ability was Ti (or Ri) plasmid-specific. Nopaline strains of A. tumefaciens and both agropine and mannopine A. rhizogenes strains efficiently transferred MSV DNA to maize. A number of strains were capable of MSV DNA transfer to other members of the Gramineae, providing information which may be essential for Agrobacterium-mediated transformation of monocotyledonous plants.

Original languageEnglish (US)
Pages (from-to)31-40
Number of pages10
JournalPlant Molecular Biology
Volume12
Issue number1
DOIs
StatePublished - Jan 1 1989

Fingerprint

Maize streak virus
Agrobacterium
Poaceae
Viruses
Zea mays
transfer DNA
DNA
corn
agropine
nopaline
Rhizobium rhizogenes
Agrobacterium radiobacter
plasmids
Plasmids
Clone Cells
Genes
Genome
clones
genome

Keywords

  • Agrobacterium
  • Gramineae
  • agroinfection
  • maize
  • maize streak virus
  • strains

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae. / Boulton, Margaret I.; Buchholz, Wallace G.; Marks, Melanie S.; Markham, Peter G.; Davies, Jeffrey W.

In: Plant Molecular Biology, Vol. 12, No. 1, 01.01.1989, p. 31-40.

Research output: Contribution to journalArticle

Boulton, Margaret I. ; Buchholz, Wallace G. ; Marks, Melanie S. ; Markham, Peter G. ; Davies, Jeffrey W. / Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae. In: Plant Molecular Biology. 1989 ; Vol. 12, No. 1. pp. 31-40.
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