Specificity and timing of neocortical transcriptome changes in response to BDNF gene ablation during embryogenesis or adulthood

C. Glorioso, M. Sabatini, T. Unger, T. Hashimoto, L. M. Monteggia, D. A. Lewis, K. Mirnics

Research output: Contribution to journalArticle

69 Scopus citations


Brain-derived neurotrophic factor (BDNF) has been reported to be critical for the development of cortical inhibitory neurons. However, the effect of BDNF on the expression of transcripts whose protein products are involved in gamma amino butric acid (GABA) neurotransmission has not been assessed. In this study, gene expression profiling using oligonucleotide microarrays was performed in prefrontal cortical tissue from mice with inducible deletions of BDNF. Both embryonic and adulthood ablation of BDNF gave rise to many shared transcriptome changes. BDNF appeared to be required to maintain gene expression in the SST-NPY-TAC1 subclass of GABA neurons, although the absence of BDNF did not alter their general phenotype as inhibitory neurons. Furthermore, we observed expression alterations in genes encoding early-immediate genes (ARC, EGR1, EGR2, FOS, DUSP1, DUSP6) and critical cellular signaling systems (CDKN1c, CCND2, CAMK1g, RGS4). These BDNF-dependent gene expression changes may illuminate the biological basis for transcriptome changes observed in certain human brain disorders.

Original languageEnglish (US)
Pages (from-to)633-648
Number of pages16
JournalMolecular Psychiatry
Issue number7
StatePublished - Jul 24 2006



  • BDNF
  • DNA microarray
  • Gene expression
  • In situ hybridization
  • Neuropeptide Y
  • RGS4
  • RT qPCR
  • Somatostatin
  • Tachykinin 1

ASJC Scopus subject areas

  • Molecular Biology
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience

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