Specificity and sensitivity of immunohistochemical detection of factor VIII/von Willebrand factor antigen in formalin-fixed paraffin-embedded tissue

Rodney D McComb, T. R. Jones, S. V. Pizzo, D. D. Bigner

Research output: Contribution to journalArticle

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Abstract

The immunohistochemical detection of factor VIII/von Willebrand factor antigen (FVIII/vWF-AG) in formalin-fixed paraffin-embedded tissues was investigated using the peroxidase-antiperoxidase (PAP) method. Highly purified human FVIII/vWF was used to raise rabbit anti-FVIII/vWF-AG serum. In addition to anti-FVIII/vWF-AG activity, the unabsorbed antiserum had anti-IgG, anti-IgM, and anti-α2-macroglobulin specificities. Following exhaustive absorption with these proteins, the antiserum reacted monospecifically for FVIII/vWF-AG in immunodiffusion, immunoelectrophoresis, and PAP immunohistochemistry. Sections of normal tissues from six patients and a total of 43 neoplasms were examined. Treatment of the tissue sections with trypsin prior to application of the antiserum markedly increased the sensitivity of FVIII/vWF-AG detection. The positive staining for FVIII/vWF-AG was restricted to endothelial cells, in both neoplastic and nonneoplastic tissue. In general, the hyperplastic endothelia in neoplastic and reactive tissues stained more intensely than those in normal tissues. Expression of FVIII/vWF-AG by nonendothelial neoplastic cells was not observed. FVIII/vWF-AG is a reliable marker for endothelial cells.

Original languageEnglish (US)
Pages (from-to)371-377
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume30
Issue number4
DOIs
StatePublished - Jan 1 1982

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Paraffin
Formaldehyde
Sensitivity and Specificity
Immune Sera
Peroxidase
Endothelial Cells
Macroglobulins
Immunoelectrophoresis
factor VIII, von Willebrand factor drug combination
Von Willebrand antigen
Immunodiffusion
Trypsin
Endothelium
Immunohistochemistry
Staining and Labeling
Rabbits
Serum
Neoplasms
Proteins

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Specificity and sensitivity of immunohistochemical detection of factor VIII/von Willebrand factor antigen in formalin-fixed paraffin-embedded tissue. / McComb, Rodney D; Jones, T. R.; Pizzo, S. V.; Bigner, D. D.

In: Journal of Histochemistry and Cytochemistry, Vol. 30, No. 4, 01.01.1982, p. 371-377.

Research output: Contribution to journalArticle

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