Specific Transfection of Inflamed Brain by Macrophages: A New Therapeutic Strategy for Neurodegenerative Diseases

Matthew J. Haney, Yuling Zhao, Emily B. Harrison, Vivek Mahajan, Shaheen Ahmed, Zhijian He, Poornima Suresh, Shawn D. Hingtgen, Natalia L. Klyachko, R Lee Mosley, Howard Eliot Gendelman, Alexander V. Kabanov, Elena V. Batrakova

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The ability to precisely upregulate genes in inflamed brain holds great therapeutic promise. Here we report a novel class of vectors, genetically modified macrophages that carry reporter and therapeutic genes to neural cells. Systemic administration of macrophages transfected ex vivo with a plasmid DNA (pDNA) encoding a potent antioxidant enzyme, catalase, produced month-long expression levels of catalase in the brain resulting in three-fold reductions in inflammation and complete neuroprotection in mouse models of Parkinson's disease (PD). This resulted in significant improvements in motor functions in PD mice. Mechanistic studies revealed that transfected macrophages secreted extracellular vesicles, exosomes, packed with catalase genetic material, pDNA and mRNA, active catalase, and NF-κb, a transcription factor involved in the encoded gene expression. Exosomes efficiently transfer their contents to contiguous neurons resulting in de novo protein synthesis in target cells. Thus, genetically modified macrophages serve as a highly efficient system for reproduction, packaging, and targeted gene and drug delivery to treat inflammatory and neurodegenerative disorders.

Original languageEnglish (US)
Article numbere61852
JournalPloS one
Volume8
Issue number4
DOIs
StatePublished - Apr 19 2013

Fingerprint

Neurodegenerative diseases
Macrophages
neurodegenerative diseases
transfection
Neurodegenerative Diseases
Catalase
Transfection
Brain
catalase
macrophages
exosomes
Exosomes
brain
therapeutics
Genes
Parkinson disease
Parkinson Disease
plasmids
Plasmids
neurons

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Haney, M. J., Zhao, Y., Harrison, E. B., Mahajan, V., Ahmed, S., He, Z., ... Batrakova, E. V. (2013). Specific Transfection of Inflamed Brain by Macrophages: A New Therapeutic Strategy for Neurodegenerative Diseases. PloS one, 8(4), [e61852]. https://doi.org/10.1371/journal.pone.0061852

Specific Transfection of Inflamed Brain by Macrophages : A New Therapeutic Strategy for Neurodegenerative Diseases. / Haney, Matthew J.; Zhao, Yuling; Harrison, Emily B.; Mahajan, Vivek; Ahmed, Shaheen; He, Zhijian; Suresh, Poornima; Hingtgen, Shawn D.; Klyachko, Natalia L.; Mosley, R Lee; Gendelman, Howard Eliot; Kabanov, Alexander V.; Batrakova, Elena V.

In: PloS one, Vol. 8, No. 4, e61852, 19.04.2013.

Research output: Contribution to journalArticle

Haney, MJ, Zhao, Y, Harrison, EB, Mahajan, V, Ahmed, S, He, Z, Suresh, P, Hingtgen, SD, Klyachko, NL, Mosley, RL, Gendelman, HE, Kabanov, AV & Batrakova, EV 2013, 'Specific Transfection of Inflamed Brain by Macrophages: A New Therapeutic Strategy for Neurodegenerative Diseases', PloS one, vol. 8, no. 4, e61852. https://doi.org/10.1371/journal.pone.0061852
Haney, Matthew J. ; Zhao, Yuling ; Harrison, Emily B. ; Mahajan, Vivek ; Ahmed, Shaheen ; He, Zhijian ; Suresh, Poornima ; Hingtgen, Shawn D. ; Klyachko, Natalia L. ; Mosley, R Lee ; Gendelman, Howard Eliot ; Kabanov, Alexander V. ; Batrakova, Elena V. / Specific Transfection of Inflamed Brain by Macrophages : A New Therapeutic Strategy for Neurodegenerative Diseases. In: PloS one. 2013 ; Vol. 8, No. 4.
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