Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes: A preliminary communication

Jean Gupta, Howard E. Gendelman, Zohreh Naghashfar, Prabodh Gupta, Neil Rosenshein, Edward Sawada, J. Donald Woodruff, Keerti Shah

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Cervical Papanicolaou smears and paraffin sections of biopsy specimens obtained from women attending dysplasia clinics were examined for viral DNA sequences by in situ hybridization technique using 35S-labeled cloned recombinant DNA probes of human papillomavirus (HPV) types 6, 11, and 16. These and one unrelated DNA probe complementary to measles virus RNA were labeled by nick translation using either one or two 35S-labeled nucleotides. The radiolabeled probes were reduced in size with DNase to 60–160 nucleotides. Paraffin sections and cervical smears were collected on pretreated slides, hybridized with the probes under stringent or nonstringent conditions for 50 h, and autoradiographed. Additional 'cervical specimens from the same women were examined for the presence of genus-specific papillomavirus capsid antigen by the immunoperoxidase technique. Preliminary results may be summarized as follows. The infecting virus could be identified in smears as well as in sections. Viral DNA sequences were detected only when there were condylomatous cells in the specimen and in only a proportion of the condylomatous cells. Even under stringent conditions, some specimens reacted with both HPV-6 and HPV-11. None of the specimens hybridized with HPV-16 or with the unrelated probe. In some instances, the cells did not hybridize with any of the three probes even when duplicate specimens contained frankly condylomatous, capsid antigen-positive cells. In situ hybridization of Papanicolaou smears or of tissue sections is a practical method for diagnosis and follow-up of specific papillomavirus infection using routinely collected material.

Original languageEnglish (US)
Pages (from-to)211-218
Number of pages8
JournalInternational Journal of Gynecological Pathology
Volume4
Issue number3
DOIs
StatePublished - Sep 1985

Fingerprint

Forensic Anthropology
Vaginal Smears
Paraffin
In Situ Hybridization
Communication
Human papillomavirus 11
Human papillomavirus 6
Papanicolaou Test
Human papillomavirus 16
Capsid
DNA Probes
Viral DNA
Nucleotides
Antigens
Measles virus
Papillomavirus Infections
Recombinant DNA
Deoxyribonucleases
Immunoenzyme Techniques
RNA

Keywords

  • Cervical smear
  • Cervical tissue
  • In situ hybridization
  • Papillomavirus

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Obstetrics and Gynecology

Cite this

Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes : A preliminary communication. / Gupta, Jean; Gendelman, Howard E.; Naghashfar, Zohreh; Gupta, Prabodh; Rosenshein, Neil; Sawada, Edward; Woodruff, J. Donald; Shah, Keerti.

In: International Journal of Gynecological Pathology, Vol. 4, No. 3, 09.1985, p. 211-218.

Research output: Contribution to journalArticle

Gupta, Jean ; Gendelman, Howard E. ; Naghashfar, Zohreh ; Gupta, Prabodh ; Rosenshein, Neil ; Sawada, Edward ; Woodruff, J. Donald ; Shah, Keerti. / Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes : A preliminary communication. In: International Journal of Gynecological Pathology. 1985 ; Vol. 4, No. 3. pp. 211-218.
@article{801266ee56cf4ca5aaada902d8fb5bdd,
title = "Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes: A preliminary communication",
abstract = "Cervical Papanicolaou smears and paraffin sections of biopsy specimens obtained from women attending dysplasia clinics were examined for viral DNA sequences by in situ hybridization technique using 35S-labeled cloned recombinant DNA probes of human papillomavirus (HPV) types 6, 11, and 16. These and one unrelated DNA probe complementary to measles virus RNA were labeled by nick translation using either one or two 35S-labeled nucleotides. The radiolabeled probes were reduced in size with DNase to 60–160 nucleotides. Paraffin sections and cervical smears were collected on pretreated slides, hybridized with the probes under stringent or nonstringent conditions for 50 h, and autoradiographed. Additional 'cervical specimens from the same women were examined for the presence of genus-specific papillomavirus capsid antigen by the immunoperoxidase technique. Preliminary results may be summarized as follows. The infecting virus could be identified in smears as well as in sections. Viral DNA sequences were detected only when there were condylomatous cells in the specimen and in only a proportion of the condylomatous cells. Even under stringent conditions, some specimens reacted with both HPV-6 and HPV-11. None of the specimens hybridized with HPV-16 or with the unrelated probe. In some instances, the cells did not hybridize with any of the three probes even when duplicate specimens contained frankly condylomatous, capsid antigen-positive cells. In situ hybridization of Papanicolaou smears or of tissue sections is a practical method for diagnosis and follow-up of specific papillomavirus infection using routinely collected material.",
keywords = "Cervical smear, Cervical tissue, In situ hybridization, Papillomavirus",
author = "Jean Gupta and Gendelman, {Howard E.} and Zohreh Naghashfar and Prabodh Gupta and Neil Rosenshein and Edward Sawada and Woodruff, {J. Donald} and Keerti Shah",
year = "1985",
month = "9",
doi = "10.1097/00004347-198509000-00005",
language = "English (US)",
volume = "4",
pages = "211--218",
journal = "International Journal of Gynecological Pathology",
issn = "0277-1691",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes

T2 - A preliminary communication

AU - Gupta, Jean

AU - Gendelman, Howard E.

AU - Naghashfar, Zohreh

AU - Gupta, Prabodh

AU - Rosenshein, Neil

AU - Sawada, Edward

AU - Woodruff, J. Donald

AU - Shah, Keerti

PY - 1985/9

Y1 - 1985/9

N2 - Cervical Papanicolaou smears and paraffin sections of biopsy specimens obtained from women attending dysplasia clinics were examined for viral DNA sequences by in situ hybridization technique using 35S-labeled cloned recombinant DNA probes of human papillomavirus (HPV) types 6, 11, and 16. These and one unrelated DNA probe complementary to measles virus RNA were labeled by nick translation using either one or two 35S-labeled nucleotides. The radiolabeled probes were reduced in size with DNase to 60–160 nucleotides. Paraffin sections and cervical smears were collected on pretreated slides, hybridized with the probes under stringent or nonstringent conditions for 50 h, and autoradiographed. Additional 'cervical specimens from the same women were examined for the presence of genus-specific papillomavirus capsid antigen by the immunoperoxidase technique. Preliminary results may be summarized as follows. The infecting virus could be identified in smears as well as in sections. Viral DNA sequences were detected only when there were condylomatous cells in the specimen and in only a proportion of the condylomatous cells. Even under stringent conditions, some specimens reacted with both HPV-6 and HPV-11. None of the specimens hybridized with HPV-16 or with the unrelated probe. In some instances, the cells did not hybridize with any of the three probes even when duplicate specimens contained frankly condylomatous, capsid antigen-positive cells. In situ hybridization of Papanicolaou smears or of tissue sections is a practical method for diagnosis and follow-up of specific papillomavirus infection using routinely collected material.

AB - Cervical Papanicolaou smears and paraffin sections of biopsy specimens obtained from women attending dysplasia clinics were examined for viral DNA sequences by in situ hybridization technique using 35S-labeled cloned recombinant DNA probes of human papillomavirus (HPV) types 6, 11, and 16. These and one unrelated DNA probe complementary to measles virus RNA were labeled by nick translation using either one or two 35S-labeled nucleotides. The radiolabeled probes were reduced in size with DNase to 60–160 nucleotides. Paraffin sections and cervical smears were collected on pretreated slides, hybridized with the probes under stringent or nonstringent conditions for 50 h, and autoradiographed. Additional 'cervical specimens from the same women were examined for the presence of genus-specific papillomavirus capsid antigen by the immunoperoxidase technique. Preliminary results may be summarized as follows. The infecting virus could be identified in smears as well as in sections. Viral DNA sequences were detected only when there were condylomatous cells in the specimen and in only a proportion of the condylomatous cells. Even under stringent conditions, some specimens reacted with both HPV-6 and HPV-11. None of the specimens hybridized with HPV-16 or with the unrelated probe. In some instances, the cells did not hybridize with any of the three probes even when duplicate specimens contained frankly condylomatous, capsid antigen-positive cells. In situ hybridization of Papanicolaou smears or of tissue sections is a practical method for diagnosis and follow-up of specific papillomavirus infection using routinely collected material.

KW - Cervical smear

KW - Cervical tissue

KW - In situ hybridization

KW - Papillomavirus

UR - http://www.scopus.com/inward/record.url?scp=0022346890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022346890&partnerID=8YFLogxK

U2 - 10.1097/00004347-198509000-00005

DO - 10.1097/00004347-198509000-00005

M3 - Article

C2 - 2997054

AN - SCOPUS:0022346890

VL - 4

SP - 211

EP - 218

JO - International Journal of Gynecological Pathology

JF - International Journal of Gynecological Pathology

SN - 0277-1691

IS - 3

ER -