Specific fragmentation of T7 phage DNA at low-melting sites

V. M. Pavlov, Yu L.L. Lyubchenko, A. S. Borovik, Yu S. Lazurkin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A method, has been developed for selective fragmentation of T7 DNA at AT-rich regions. The molecules have been subjected to complete digestion with single-strand-specific SI endonuolease after fixation of DNA AT-rich regions in the denatured state by glyoxal. The treatment resulted in three fragments having molecular weights of 13.6±0.4, 8.2±0.4 and 3.5±0.16 megadaltons as determined by electron microscopy. The position of these fragments along the T7 DNA molecule has been determined by means of analysis of the intermediates during SI-cleavage.

Original languageEnglish (US)
Pages (from-to)4053-4062
Number of pages10
JournalNucleic acids research
Volume4
Issue number11
DOIs
StatePublished - Nov 1 1977

Fingerprint

AT Rich Sequence
Bacteriophage T7
Bacteriophages
Fragmentation
Melting
Freezing
Fragment
DNA
Molecules
Glyoxal
Electron Microscopy
Fixation
DNA Fragmentation
Digestion
Molecular Weight
Electron microscopy
Molecular weight

ASJC Scopus subject areas

  • Genetics

Cite this

Specific fragmentation of T7 phage DNA at low-melting sites. / Pavlov, V. M.; Lyubchenko, Yu L.L.; Borovik, A. S.; Lazurkin, Yu S.

In: Nucleic acids research, Vol. 4, No. 11, 01.11.1977, p. 4053-4062.

Research output: Contribution to journalArticle

Pavlov, V. M. ; Lyubchenko, Yu L.L. ; Borovik, A. S. ; Lazurkin, Yu S. / Specific fragmentation of T7 phage DNA at low-melting sites. In: Nucleic acids research. 1977 ; Vol. 4, No. 11. pp. 4053-4062.
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