Specific expression in reproductive tissues and fate of a mitochondrial sterility-associated protein in cytoplasmic male-sterile bean

Andre R. Abad, Brian J. Mehrtens, Sally Mackenzie

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

In common bean, cytoplasmic male sterility has been associated with a unique sequence found in the mitochondrial genome, designated pvs (for Phaseolus vulgaris sterility sequence). Within the pvs sequence two open reading frames are encoded, ORF98 and ORF239. We have raised rabbit polyclonal antibodies against Pvs-ORF239 to evaluate the role of this putative male sterility-associated protein. Histological investigation of pollen development revealed that in the male-sterile bean line, callose deposition was abnormal and microspores remained as tetrads as previously reported. Pvs-ORF239 was found to be localized within the reproductive tissues of the male-sterile bean line, in con rast to all other cytoplasmic male sterility systems studied to date. This protein was associated with mitochondna, the callose layer, and developing primary cell walls during microsporogenesis. Expression of pvs-orf239 was not detected in fertile plants containing restorer gene Fr2. These observations, together with previous reports, suggest that nuclear restorer gene Fr2 interferes with expression of the pvs region post-transcriptionally.

Original languageEnglish (US)
Pages (from-to)271-285
Number of pages15
JournalPlant Cell
Volume7
Issue number3
StatePublished - Mar 1 1995

Fingerprint

Phaseolus
Infertility
Phaseolus vulgaris
Male Infertility
beans
Genes
Tissue
cytoplasmic male sterility
callose
Proteins
Plant Gametogenesis
proteins
microsporogenesis
Mitochondrial Genome
male fertility
microspores
Pollen
polyclonal antibodies
Cells
Cell Wall

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Specific expression in reproductive tissues and fate of a mitochondrial sterility-associated protein in cytoplasmic male-sterile bean. / Abad, Andre R.; Mehrtens, Brian J.; Mackenzie, Sally.

In: Plant Cell, Vol. 7, No. 3, 01.03.1995, p. 271-285.

Research output: Contribution to journalArticle

Abad, Andre R. ; Mehrtens, Brian J. ; Mackenzie, Sally. / Specific expression in reproductive tissues and fate of a mitochondrial sterility-associated protein in cytoplasmic male-sterile bean. In: Plant Cell. 1995 ; Vol. 7, No. 3. pp. 271-285.
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