Specific down-regulation of annexin II expression in human cells interferes with cell proliferation

Yungping Chiang, A Angie Rizzino, Zita A. Sibenaller, Marc S. Wold, Jamboor K. Vishwanatha

Research output: Contribution to journalArticle

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Abstract

The protein-tyrosine kinase substrate annexin II is a growth regulated gene whose expression is increased in several human cancers. While the precise function of this protein is not understood, annexin II is proposed to be involved in multiple physiological activities, including DNA synthesis and cell proliferation. Targeted disruption of the annexin II gene affects calcium signaling, tyrosine phosphorylation and apoptosis, indicating the important physiological role of this protein. We used a transient co-transfection assay to regulate annexin II expression in human HeLa, 293 and 293T cells, and measured the effects of annexin II down regulation on DNA synthesis and proliferation. Transfection of cells with an antisense annexin II vector results in inhibition of cell division and proliferation, with concomitant reduction in annexin II message and protein levels. Cellular DNA synthesis is significantly reduced in antisense transfected cells. Replication extracts made from antisense transfected cells have significantly reduced efficiency to support SV40 in vitro DNA replication, while the extracts made from sense transfected cells are fully capable of replication. Our results indicate an important role of annexin II in cellular DNA synthesis and cell proliferation.

Original languageEnglish (US)
Pages (from-to)139-147
Number of pages9
JournalMolecular and Cellular Biochemistry
Volume199
Issue number1-2
DOIs
StatePublished - Oct 21 1999

Fingerprint

Annexin A2
Cell proliferation
Down-Regulation
Cells
Cell Proliferation
DNA
Transfection
Phosphorylation
Proteins
Calcium Signaling
HEK293 Cells
DNA Replication
HeLa Cells
Gene expression
Cell Division
Protein-Tyrosine Kinases
Tyrosine
Assays
Genes
Apoptosis

Keywords

  • Annexin II
  • Antisense
  • Cell proliferation
  • DNA synthesis
  • Simian virus 40

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Specific down-regulation of annexin II expression in human cells interferes with cell proliferation. / Chiang, Yungping; Rizzino, A Angie; Sibenaller, Zita A.; Wold, Marc S.; Vishwanatha, Jamboor K.

In: Molecular and Cellular Biochemistry, Vol. 199, No. 1-2, 21.10.1999, p. 139-147.

Research output: Contribution to journalArticle

Chiang, Yungping ; Rizzino, A Angie ; Sibenaller, Zita A. ; Wold, Marc S. ; Vishwanatha, Jamboor K. / Specific down-regulation of annexin II expression in human cells interferes with cell proliferation. In: Molecular and Cellular Biochemistry. 1999 ; Vol. 199, No. 1-2. pp. 139-147.
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