Specific binding of poly(ADP-ribose) polymerase-1 to cruciform hairpins

Vladimir N. Potaman, Luda S. Shlyakhtenko, Elena A. Oussatcheva, Yuri L Lyubchenko, Viatcheslav A. Soldatenkov

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Poly(ADP-ribose) polymerase-1 (PARP-1) participates in DNA cleavage and rejoining-dependent reactions, such as DNA replication, recombination and repair. PARP-1 is also important in transcriptional regulation, although the determinants for its binding to undamaged genomic DNA have not been defined. Previously, we have shown by low-resolution mapping that PARP-1 may bind to the cruciform-forming regions of its own promoter. Here, using DNase I and nuclease P1 footprinting and atomic force microscopy, we show that PARP-1 binds to stem/loop boundaries of cruciform hairpins. Cleavage of the cruciform by the junction resolvase T4 endonuclease VII is independent of PARP-1, which indicates that PARP-1 does not bind to the four-arm junctions of the cruciform. Thus, PARP-1 differs from other cruciform-binding proteins by binding to hairpin tips rather than to junctions. Furthermore, our data indicate that PARP-1 can interact with the gene regulatory sequences by binding to the promoter-localized cruciforms.

Original languageEnglish (US)
Pages (from-to)609-615
Number of pages7
JournalJournal of Molecular Biology
Volume348
Issue number3
DOIs
StatePublished - May 6 2005

Fingerprint

Recombinational DNA Repair
DNA Cleavage
Recombinases
Poly (ADP-Ribose) Polymerase-1
Atomic Force Microscopy
Deoxyribonuclease I
Regulator Genes
DNA Replication
Protein Binding
DNA Repair
Carrier Proteins
DNA
endodeoxyribonuclease VII

Keywords

  • Cruciform hairpin
  • DNA-binding
  • Footprint
  • PARP-1
  • Transcriptional regulation

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Potaman, V. N., Shlyakhtenko, L. S., Oussatcheva, E. A., Lyubchenko, Y. L., & Soldatenkov, V. A. (2005). Specific binding of poly(ADP-ribose) polymerase-1 to cruciform hairpins. Journal of Molecular Biology, 348(3), 609-615. https://doi.org/10.1016/j.jmb.2005.03.010

Specific binding of poly(ADP-ribose) polymerase-1 to cruciform hairpins. / Potaman, Vladimir N.; Shlyakhtenko, Luda S.; Oussatcheva, Elena A.; Lyubchenko, Yuri L; Soldatenkov, Viatcheslav A.

In: Journal of Molecular Biology, Vol. 348, No. 3, 06.05.2005, p. 609-615.

Research output: Contribution to journalArticle

Potaman, VN, Shlyakhtenko, LS, Oussatcheva, EA, Lyubchenko, YL & Soldatenkov, VA 2005, 'Specific binding of poly(ADP-ribose) polymerase-1 to cruciform hairpins', Journal of Molecular Biology, vol. 348, no. 3, pp. 609-615. https://doi.org/10.1016/j.jmb.2005.03.010
Potaman, Vladimir N. ; Shlyakhtenko, Luda S. ; Oussatcheva, Elena A. ; Lyubchenko, Yuri L ; Soldatenkov, Viatcheslav A. / Specific binding of poly(ADP-ribose) polymerase-1 to cruciform hairpins. In: Journal of Molecular Biology. 2005 ; Vol. 348, No. 3. pp. 609-615.
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