Spatiotemporal Characterizations of Spontaneously Beating Cardiomyocytes with Adaptive Reference Digital Image Correlation

Akankshya Shradhanjali, Brandon D. Riehl, Bin Duan, Ruiguo Yang, Jung Yul Lim

Research output: Contribution to journalArticle

Abstract

We developed an Adaptive Reference-Digital Image Correlation (AR-DIC) method that enables unbiased and accurate mechanics measurements of moving biological tissue samples. We applied the AR-DIC analysis to a spontaneously beating cardiomyocyte (CM) tissue, and could provide correct quantifications of tissue displacement and strain for the beating CMs utilizing physiologically-relevant, sarcomere displacement length-based contraction criteria. The data were further synthesized into novel spatiotemporal parameters of CM contraction to account for the CM beating homogeneity, synchronicity, and propagation as holistic measures of functional myocardial tissue development. Our AR-DIC analyses may thus provide advanced non-invasive characterization tools for assessing the development of spontaneously contracting CMs, suggesting an applicability in myocardial regenerative medicine.

Original languageEnglish (US)
Article number18382
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Cardiac Myocytes
Sarcomeres
Regenerative Medicine
Mechanics

ASJC Scopus subject areas

  • General

Cite this

Spatiotemporal Characterizations of Spontaneously Beating Cardiomyocytes with Adaptive Reference Digital Image Correlation. / Shradhanjali, Akankshya; Riehl, Brandon D.; Duan, Bin; Yang, Ruiguo; Lim, Jung Yul.

In: Scientific reports, Vol. 9, No. 1, 18382, 01.12.2019.

Research output: Contribution to journalArticle

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