SPAF, a new AAA-protein specific to early spermatogenesis and malignant conversion

Yuangang Liu, Jennifer Black, Nicholas Kisiel, Molly F. Kulesz-Martin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A novel spermatogenesis associated factor (SPAF) was found to be aberrantly expressed at the malignant conversion stage in a clonal epidermal model of chemical carcinogenesis. Sequence analysis revealed two ATPase modules, classifying this gene as a new member of the AAA-protein family (ATPase associated with diverse activities). Immunohistochemical staining of mouse testis sections with SPAF antibody localized expression to spermatogonia and early spermatocytes in the basal compartment of the seminiferous tubules. Northern and Western analysis of SPAF expression in testes of mice at different developmental stages confirmed its expression at early stages of spermatogenesis. In view of a mitochondrial-localization-like signal, sequence similarities to membrane-associated proteins, ATP binding properties, and intracellular expression patterns in testis, we speculate that SPAF protein may be involved in morphological and functional mitochondrial transformations during spermatogenesis. Ectopic expression of the SPAF gent in malignant epidermal cells may signify adoption of an early germ cell-like phenotype advantageous in malignant conversion.

Original languageEnglish (US)
Pages (from-to)1579-1588
Number of pages10
JournalOncogene
Volume19
Issue number12
DOIs
StatePublished - Mar 16 2000

Fingerprint

Spermatogenesis
Testis
Proteins
Adenosine Triphosphatases
Chemical Models
Spermatogonia
Seminiferous Tubules
Spermatocytes
Gene Regulatory Networks
Protein Sorting Signals
Protein Binding
Germ Cells
Sequence Analysis
Membrane Proteins
Carcinogenesis
Adenosine Triphosphate
spermatogenesis associated factor
Staining and Labeling
Phenotype
Antibodies

Keywords

  • AAA-protein
  • Carcinogenesis
  • Mitochondria
  • SPAF
  • Spermatogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

SPAF, a new AAA-protein specific to early spermatogenesis and malignant conversion. / Liu, Yuangang; Black, Jennifer; Kisiel, Nicholas; Kulesz-Martin, Molly F.

In: Oncogene, Vol. 19, No. 12, 16.03.2000, p. 1579-1588.

Research output: Contribution to journalArticle

Liu, Yuangang ; Black, Jennifer ; Kisiel, Nicholas ; Kulesz-Martin, Molly F. / SPAF, a new AAA-protein specific to early spermatogenesis and malignant conversion. In: Oncogene. 2000 ; Vol. 19, No. 12. pp. 1579-1588.
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