Southern blot analysis of BII cell line - A putative variant of HL-60

Hamid Band, V. Corredor, J. Lustgarten, K. Huebner, J. D. Griffin, D. W. Kufe, E. J. Yunis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Southern blot analysis of various genes was used to compare the human promyelocytic leukemia cell line HL-60 and the BII cell line, which reportedly arose as a spontaneous differentiation inducer-resistant variant from an HL-60 culture. Granulocyte-macrophage colony stimulating factor gene restriction fragment polymorphism, due to a partial deletion of one of the alleles of this gene in HL-60, was not observed in the BII cells. Furthermore, the p53 oncogene, most of which is deleted in the HL-60 cell line, was found to be intact in the BII cell line. Human leukocyte antigen typing revealed that the two cell lines shared the A locus but differed at the B locus. Several unique restriction fragments hybridizing to human leukocyte antigen class I and DR beta gene probes were observed in the DNA digests of each cell line. Altogether these data provide definitive evidence that BII represents a human cell line of different origin than HL-60. Further lineage determination of this cell could add a useful member to the group of leukemic cell lines.

Original languageEnglish (US)
Pages (from-to)142-145
Number of pages4
JournalLeukemia
Volume1
Issue number2
StatePublished - Dec 1 1987

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Southern Blotting
Cell Line
HL-60 Cells
HLA Antigens
Genes
Granulocyte-Macrophage Colony-Stimulating Factor
Oncogenes
Leukemia
Alleles
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Band, H., Corredor, V., Lustgarten, J., Huebner, K., Griffin, J. D., Kufe, D. W., & Yunis, E. J. (1987). Southern blot analysis of BII cell line - A putative variant of HL-60. Leukemia, 1(2), 142-145.

Southern blot analysis of BII cell line - A putative variant of HL-60. / Band, Hamid; Corredor, V.; Lustgarten, J.; Huebner, K.; Griffin, J. D.; Kufe, D. W.; Yunis, E. J.

In: Leukemia, Vol. 1, No. 2, 01.12.1987, p. 142-145.

Research output: Contribution to journalArticle

Band, H, Corredor, V, Lustgarten, J, Huebner, K, Griffin, JD, Kufe, DW & Yunis, EJ 1987, 'Southern blot analysis of BII cell line - A putative variant of HL-60', Leukemia, vol. 1, no. 2, pp. 142-145.
Band H, Corredor V, Lustgarten J, Huebner K, Griffin JD, Kufe DW et al. Southern blot analysis of BII cell line - A putative variant of HL-60. Leukemia. 1987 Dec 1;1(2):142-145.
Band, Hamid ; Corredor, V. ; Lustgarten, J. ; Huebner, K. ; Griffin, J. D. ; Kufe, D. W. ; Yunis, E. J. / Southern blot analysis of BII cell line - A putative variant of HL-60. In: Leukemia. 1987 ; Vol. 1, No. 2. pp. 142-145.
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