286 Citations (Scopus)

Abstract

Purpose: We investigated the contribution of Sonic hedgehog (SHH) to pancreatic cancer progression. Experimental Design: We expressed SHH in a transformed primary ductal-derived epithelial cell line from the human pancreas, transformed hTert-HPNE (T-HPNE), and evaluated the effects on tumor growth. We also directly inhibited the activity of SHH in vivo by administering a blocking antibody to mice challenged orthotopically with the Capan-2 pancreatic cancer cell line, which is known to express SHH and form moderately differentiated tumors in nude mice. Results: Our data provide evidence that expression of SHH influences tumor growth by contributing to the formation of desmoplasia in pancreatic cancer. We further show that SHH affects the differentiation and motility of human pancreatic stellate cells and fibroblasts. Conclusions: These data suggest that SHH contributes to the formation of desmoplasia in pancreatic cancer, an important component of the tumor microenvironment.

Original languageEnglish (US)
Pages (from-to)5995-6004
Number of pages10
JournalClinical Cancer Research
Volume14
Issue number19
DOIs
StatePublished - Oct 1 2008

Fingerprint

Hedgehogs
Pancreatic Neoplasms
Pancreatic Stellate Cells
Cell Line
Neoplasms
Blocking Antibodies
Tumor Microenvironment
Growth
Nude Mice
Pancreas
Research Design
Fibroblasts
Epithelial Cells

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Sonic hedgehog promotes desmoplasia in pancreatic cancer. / Bailey, Jennifer M.; Swanson, Benjamin J; Hamada, Tomofumi; Eggers, John P.; Singh, Pankaj; Caffery, Thomas; Ouellette, Michel M; Hollingsworth, Michael A.

In: Clinical Cancer Research, Vol. 14, No. 19, 01.10.2008, p. 5995-6004.

Research output: Contribution to journalArticle

Bailey, Jennifer M. ; Swanson, Benjamin J ; Hamada, Tomofumi ; Eggers, John P. ; Singh, Pankaj ; Caffery, Thomas ; Ouellette, Michel M ; Hollingsworth, Michael A. / Sonic hedgehog promotes desmoplasia in pancreatic cancer. In: Clinical Cancer Research. 2008 ; Vol. 14, No. 19. pp. 5995-6004.
@article{a6889c7fce254ecf9c50ba9cd723effa,
title = "Sonic hedgehog promotes desmoplasia in pancreatic cancer",
abstract = "Purpose: We investigated the contribution of Sonic hedgehog (SHH) to pancreatic cancer progression. Experimental Design: We expressed SHH in a transformed primary ductal-derived epithelial cell line from the human pancreas, transformed hTert-HPNE (T-HPNE), and evaluated the effects on tumor growth. We also directly inhibited the activity of SHH in vivo by administering a blocking antibody to mice challenged orthotopically with the Capan-2 pancreatic cancer cell line, which is known to express SHH and form moderately differentiated tumors in nude mice. Results: Our data provide evidence that expression of SHH influences tumor growth by contributing to the formation of desmoplasia in pancreatic cancer. We further show that SHH affects the differentiation and motility of human pancreatic stellate cells and fibroblasts. Conclusions: These data suggest that SHH contributes to the formation of desmoplasia in pancreatic cancer, an important component of the tumor microenvironment.",
author = "Bailey, {Jennifer M.} and Swanson, {Benjamin J} and Tomofumi Hamada and Eggers, {John P.} and Pankaj Singh and Thomas Caffery and Ouellette, {Michel M} and Hollingsworth, {Michael A}",
year = "2008",
month = "10",
day = "1",
doi = "10.1158/1078-0432.CCR-08-0291",
language = "English (US)",
volume = "14",
pages = "5995--6004",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "19",

}

TY - JOUR

T1 - Sonic hedgehog promotes desmoplasia in pancreatic cancer

AU - Bailey, Jennifer M.

AU - Swanson, Benjamin J

AU - Hamada, Tomofumi

AU - Eggers, John P.

AU - Singh, Pankaj

AU - Caffery, Thomas

AU - Ouellette, Michel M

AU - Hollingsworth, Michael A

PY - 2008/10/1

Y1 - 2008/10/1

N2 - Purpose: We investigated the contribution of Sonic hedgehog (SHH) to pancreatic cancer progression. Experimental Design: We expressed SHH in a transformed primary ductal-derived epithelial cell line from the human pancreas, transformed hTert-HPNE (T-HPNE), and evaluated the effects on tumor growth. We also directly inhibited the activity of SHH in vivo by administering a blocking antibody to mice challenged orthotopically with the Capan-2 pancreatic cancer cell line, which is known to express SHH and form moderately differentiated tumors in nude mice. Results: Our data provide evidence that expression of SHH influences tumor growth by contributing to the formation of desmoplasia in pancreatic cancer. We further show that SHH affects the differentiation and motility of human pancreatic stellate cells and fibroblasts. Conclusions: These data suggest that SHH contributes to the formation of desmoplasia in pancreatic cancer, an important component of the tumor microenvironment.

AB - Purpose: We investigated the contribution of Sonic hedgehog (SHH) to pancreatic cancer progression. Experimental Design: We expressed SHH in a transformed primary ductal-derived epithelial cell line from the human pancreas, transformed hTert-HPNE (T-HPNE), and evaluated the effects on tumor growth. We also directly inhibited the activity of SHH in vivo by administering a blocking antibody to mice challenged orthotopically with the Capan-2 pancreatic cancer cell line, which is known to express SHH and form moderately differentiated tumors in nude mice. Results: Our data provide evidence that expression of SHH influences tumor growth by contributing to the formation of desmoplasia in pancreatic cancer. We further show that SHH affects the differentiation and motility of human pancreatic stellate cells and fibroblasts. Conclusions: These data suggest that SHH contributes to the formation of desmoplasia in pancreatic cancer, an important component of the tumor microenvironment.

UR - http://www.scopus.com/inward/record.url?scp=58149143021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149143021&partnerID=8YFLogxK

U2 - 10.1158/1078-0432.CCR-08-0291

DO - 10.1158/1078-0432.CCR-08-0291

M3 - Article

VL - 14

SP - 5995

EP - 6004

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 19

ER -