Solution structure of ZipA, a crucial component of Escherichia coli cell division

F. J. Moy, E. Glasfeld, L. Mosyak, R. Powers

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

ZipA, an essential component of cell division in Escherichia coli, interacts with the FtsZ protein at the midcell in one of the initial steps of septum formation. The high-resolution solution structure of the 144-residue C-terminal domain of E. coli ZipA (ZipA185-328) has been determined by multidimensional heteronuclear NMR. A total of 30 structures were calculated by means of hybrid distance geometry-simulated annealing using a total of 2758 experimental NMR restraints. The atomic root means square distribution about the mean coordinate positions for residues 6-142 for the 30 structures is 0.37 ± 0.04 Å for the backbone atoms, 0.78 ± 0.05 Å for all atoms, and 0.45 ± 0.04 Å for all atoms excluding disordered side chains. The NMR solution structure of ZipA185-328 is composed of three α-helices and a β-sheet consisting of six antiparallel β-strands where the α-helices and the β-sheet form surfaces directly opposite each other. A C-terminal peptide from FtsZ has been shown to bind ZipA185-328 in a hydrophobic channel formed by the β-sheet providing insight into the ZipA-FtsZ interaction. An unexpected similarity between the ZipA185-328 fold and the split β-α-β fold observed in many RNA binding proteins may further our understanding of the critical ZipA-FtsZ interaction.

Original languageEnglish (US)
Pages (from-to)9146-9156
Number of pages11
JournalBiochemistry
Volume39
Issue number31
DOIs
StatePublished - Aug 8 2000

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Cell Division
Escherichia coli
Cells
Nuclear magnetic resonance
Biomolecular Nuclear Magnetic Resonance
Atoms
RNA-Binding Proteins
Simulated annealing
Peptides
Proteins
Geometry

ASJC Scopus subject areas

  • Biochemistry

Cite this

Solution structure of ZipA, a crucial component of Escherichia coli cell division. / Moy, F. J.; Glasfeld, E.; Mosyak, L.; Powers, R.

In: Biochemistry, Vol. 39, No. 31, 08.08.2000, p. 9146-9156.

Research output: Contribution to journalArticle

Moy, F. J. ; Glasfeld, E. ; Mosyak, L. ; Powers, R. / Solution structure of ZipA, a crucial component of Escherichia coli cell division. In: Biochemistry. 2000 ; Vol. 39, No. 31. pp. 9146-9156.
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