Solution structure of the Pseudomonas putida protein PpPutA45 and its DNA complex

Steven Halouska, Yuzhen Zhou, Donald F Becker, Robert Powers

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Proline utilization A (PutA) is a membrane-associated multifunctional enzyme that catalyzes the oxidation of proline to glutamate in a two-step process. In certain, gram-negative bacteria such as Pseudomonas putida, PutA also acts as an auto repressor in the cytoplasm, when an insufficient concentration of proline is available. Here, the N-terminal residues 1-45 of PutA from P. putida (PpPutA45) are shown to be responsible for DNA binding and dimerization. The solution structure of PpPutA45 was determined using NMR methods, where the protein is shown to be a symmetrical homodimer (12 kDa) consisting of two ribbon-helix-helix (RHH) structures. DNA sequence recognition by PpPutA45 was determined using DNA gel mobility shift assays and NMR chemical shift perturbations (CSPs). PpPutA45 was shown to bind a 14 base-pair DNA oligomer (5′-GCGGTTGCACCTTT-3′). A model of the PpPutA45-DNA oligomer complex was generated using Haddock 2.1. The antiparallel β-sheet that results from PpPutA45 dimerization serves as the DNA recognition binding site by inserting into the DNA major groove. The dimeric core of four α-helices provides a structural scaffold for the β-sheet from which residues Thr5, Gly7, and Lys9 make sequence-specific contacts with the DNA. The structural model implies flexibility of Lys9 which can make hydrogen bond contacts with either guanine or thymine. The high sequence and structure conservation of the PutA RHH domain suggest interdomain interactions play an important role in the evolution of the protein.

Original languageEnglish (US)
Pages (from-to)12-27
Number of pages16
JournalProteins: Structure, Function and Bioinformatics
Volume75
Issue number1
DOIs
StatePublished - Apr 1 2009

Fingerprint

Pseudomonas putida
Proline
DNA
Proteins
Dimerization
Oligomers
Multifunctional Enzymes
Nuclear magnetic resonance
Thymine
DNA sequences
Structural Models
Guanine
Chemical shift
Electrophoretic Mobility Shift Assay
Gram-Negative Bacteria
Scaffolds
Base Pairing
Glutamic Acid
Hydrogen
Assays

Keywords

  • NMR solution structure
  • Pseudomonas putida
  • PutA
  • PutA-DNA complex
  • ribbon-helix-helix (RHH) structures

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Solution structure of the Pseudomonas putida protein PpPutA45 and its DNA complex. / Halouska, Steven; Zhou, Yuzhen; Becker, Donald F; Powers, Robert.

In: Proteins: Structure, Function and Bioinformatics, Vol. 75, No. 1, 01.04.2009, p. 12-27.

Research output: Contribution to journalArticle

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