Solution Conformation of a Synthetic Fragment of Human Pituitary Growth Hormone. Two-Dimensional NMR of an α-Helical Dimer

Vikram Roongta, Robert Powers, Claude Jones, Michael J. Beakage, James E. Shields, David G. Gorenstein

Research output: Contribution to journalArticle

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Abstract

Circular dichroism and two-dimensional NMR spectra indicate that a peptide fragment consisting of the first 28 residues from the N-terminus of human growth hormone (hGH 1-28) has considerable α-helical structure. The peptide, (1) H-Phe-Pro-Thr-Ile-Pro-Leu-Ser-Arg-Leu-Phe-Asp-Asn-Ala-Met-Leu-Arg-Ala-His-Arg-Leu-His-Gln-Leu-Ala-Phe-Asp-Thr-Tyr-OH (28), was synthesized on an automated peptide synthesizer using the Merrifield solid-phase method. The peptide can be modeled as an amphiphilic helix, and the unusual stability of the α-helix in aqueous solution is suggested to be attributable to formation of a dimer of α-helices. Most of the NMR signals were assigned through pure absorption phase COSY/NOESY and single- and double-relay COSY 2D NMR spectra by using the sequential assignment methodology. The NOEs were large and negative, suggesting that the peptide was not a random coil and that it existed in solution primarily as a large, fairly rigid macromolecule, consistent with the dimer structure. A network of NαHi-NαHi+1 NOESY crosspeaks is observed from residues 13 to 18 as are several other crosspeaks which indicate that the peptide has considerable α-helical structure between residues 8 and 24. In addition, gel filtration of the peptide is consistent with a dimer structure, presumably involving packing of the two hydrophobic faces of the amphiphilic α-helices.

Original languageEnglish (US)
Pages (from-to)1048-1054
Number of pages7
JournalBiochemistry
Volume28
Issue number3
DOIs
StatePublished - Jan 1 1989

Fingerprint

Human Growth Hormone
Dimers
Growth Hormone
Conformations
Nuclear magnetic resonance
Peptides
Peptide Fragments
Circular Dichroism
Macromolecules
Gel Chromatography
Gels

ASJC Scopus subject areas

  • Biochemistry

Cite this

Solution Conformation of a Synthetic Fragment of Human Pituitary Growth Hormone. Two-Dimensional NMR of an α-Helical Dimer. / Roongta, Vikram; Powers, Robert; Jones, Claude; Beakage, Michael J.; Shields, James E.; Gorenstein, David G.

In: Biochemistry, Vol. 28, No. 3, 01.01.1989, p. 1048-1054.

Research output: Contribution to journalArticle

Roongta, Vikram ; Powers, Robert ; Jones, Claude ; Beakage, Michael J. ; Shields, James E. ; Gorenstein, David G. / Solution Conformation of a Synthetic Fragment of Human Pituitary Growth Hormone. Two-Dimensional NMR of an α-Helical Dimer. In: Biochemistry. 1989 ; Vol. 28, No. 3. pp. 1048-1054.
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