Soluble factors modulate changes in collagen gene expression in abdominal aortic aneurysms

D. J. Minion, Y. Wang, T. G. Lynch, I. J. Fox, G. D. Prorok, Bernard Timothy Baxter, W. C. Krupski, M. D. Tilson, A. W. Clowes, K. Nolan, T. K. Hunt, J. Cohen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background. Although increased procollagen gene expression and synthesis have been implicated in the progression of abdominal aortic aneurysms (AAA), factors modulating this change have not been identified. Furthermore, it is not known whether the increase in AAA procollagen expression is specific to this disease or also occurs in tissue affected by atherosclerotic occlusive disease (AOD). If paracrine rather than autocrine factors are responsible for increased gene expression in AAA, this effect should be transferable to target smooth muscle cells through conditioned media. Our objectives were to determine 1α(I) procollagen messenger RNA levels in AOD tissue compared with normal and AAA and to determine whether differences noted in tissue procollagen gene expression could be transferred through conditioned media from normal, AOD, and AAA tissues to target smooth muscle cells in primary culture. Methods. Normal, AOD, and AAA tissue was used for tissue RNA extraction or was minced and washed with serum-free media (4° C) X 30 minutes and the media applied to human aortic smooth muscle cells (SMC) in primary culture for 36 hours. Total RNA from tissue and SMC exposed to conditioned media was analyzed by Northern and dot blot analysis for 1α(I) procollagen. Results. Relative tissue 1α(I) procollagen levels were not increased in AOD (0.23 ± 0.05) as compared with normal (0.17 ± 0.03); both were decreased compared with AAA (0.53 ± 0.07; p < 0.01). The 1α(I) procollagen levels in SMC exposed to conditioned media from AAA (1.73 ± 0.15) were increased (p < 0.05) compared with AOD (1.10 ± 0.12) and normal (1.16 ± 0.16). Conclusions. There is no increase in tissue AOD procollagen gene expression. The ability to transfer the same relative patterns of gene expression from tissue to target SMC with conditioned media suggests that paracrine, rather than autocrine, factors modulate procollagen expression in AAA tissues.

Original languageEnglish (US)
Pages (from-to)252-257
Number of pages6
JournalSurgery
Volume114
Issue number2
StatePublished - Jan 1 1993

Fingerprint

Abdominal Aortic Aneurysm
Procollagen
Collagen
Gene Expression
Smooth Muscle Myocytes
Conditioned Culture Medium
RNA
Serum-Free Culture Media
Northern Blotting
Messenger RNA

ASJC Scopus subject areas

  • Surgery

Cite this

Minion, D. J., Wang, Y., Lynch, T. G., Fox, I. J., Prorok, G. D., Baxter, B. T., ... Cohen, J. (1993). Soluble factors modulate changes in collagen gene expression in abdominal aortic aneurysms. Surgery, 114(2), 252-257.

Soluble factors modulate changes in collagen gene expression in abdominal aortic aneurysms. / Minion, D. J.; Wang, Y.; Lynch, T. G.; Fox, I. J.; Prorok, G. D.; Baxter, Bernard Timothy; Krupski, W. C.; Tilson, M. D.; Clowes, A. W.; Nolan, K.; Hunt, T. K.; Cohen, J.

In: Surgery, Vol. 114, No. 2, 01.01.1993, p. 252-257.

Research output: Contribution to journalArticle

Minion, DJ, Wang, Y, Lynch, TG, Fox, IJ, Prorok, GD, Baxter, BT, Krupski, WC, Tilson, MD, Clowes, AW, Nolan, K, Hunt, TK & Cohen, J 1993, 'Soluble factors modulate changes in collagen gene expression in abdominal aortic aneurysms', Surgery, vol. 114, no. 2, pp. 252-257.
Minion DJ, Wang Y, Lynch TG, Fox IJ, Prorok GD, Baxter BT et al. Soluble factors modulate changes in collagen gene expression in abdominal aortic aneurysms. Surgery. 1993 Jan 1;114(2):252-257.
Minion, D. J. ; Wang, Y. ; Lynch, T. G. ; Fox, I. J. ; Prorok, G. D. ; Baxter, Bernard Timothy ; Krupski, W. C. ; Tilson, M. D. ; Clowes, A. W. ; Nolan, K. ; Hunt, T. K. ; Cohen, J. / Soluble factors modulate changes in collagen gene expression in abdominal aortic aneurysms. In: Surgery. 1993 ; Vol. 114, No. 2. pp. 252-257.
@article{64181f3e9527477fb1408db0f3b14469,
title = "Soluble factors modulate changes in collagen gene expression in abdominal aortic aneurysms",
abstract = "Background. Although increased procollagen gene expression and synthesis have been implicated in the progression of abdominal aortic aneurysms (AAA), factors modulating this change have not been identified. Furthermore, it is not known whether the increase in AAA procollagen expression is specific to this disease or also occurs in tissue affected by atherosclerotic occlusive disease (AOD). If paracrine rather than autocrine factors are responsible for increased gene expression in AAA, this effect should be transferable to target smooth muscle cells through conditioned media. Our objectives were to determine 1α(I) procollagen messenger RNA levels in AOD tissue compared with normal and AAA and to determine whether differences noted in tissue procollagen gene expression could be transferred through conditioned media from normal, AOD, and AAA tissues to target smooth muscle cells in primary culture. Methods. Normal, AOD, and AAA tissue was used for tissue RNA extraction or was minced and washed with serum-free media (4° C) X 30 minutes and the media applied to human aortic smooth muscle cells (SMC) in primary culture for 36 hours. Total RNA from tissue and SMC exposed to conditioned media was analyzed by Northern and dot blot analysis for 1α(I) procollagen. Results. Relative tissue 1α(I) procollagen levels were not increased in AOD (0.23 ± 0.05) as compared with normal (0.17 ± 0.03); both were decreased compared with AAA (0.53 ± 0.07; p < 0.01). The 1α(I) procollagen levels in SMC exposed to conditioned media from AAA (1.73 ± 0.15) were increased (p < 0.05) compared with AOD (1.10 ± 0.12) and normal (1.16 ± 0.16). Conclusions. There is no increase in tissue AOD procollagen gene expression. The ability to transfer the same relative patterns of gene expression from tissue to target SMC with conditioned media suggests that paracrine, rather than autocrine, factors modulate procollagen expression in AAA tissues.",
author = "Minion, {D. J.} and Y. Wang and Lynch, {T. G.} and Fox, {I. J.} and Prorok, {G. D.} and Baxter, {Bernard Timothy} and Krupski, {W. C.} and Tilson, {M. D.} and Clowes, {A. W.} and K. Nolan and Hunt, {T. K.} and J. Cohen",
year = "1993",
month = "1",
day = "1",
language = "English (US)",
volume = "114",
pages = "252--257",
journal = "Surgery (United States)",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - Soluble factors modulate changes in collagen gene expression in abdominal aortic aneurysms

AU - Minion, D. J.

AU - Wang, Y.

AU - Lynch, T. G.

AU - Fox, I. J.

AU - Prorok, G. D.

AU - Baxter, Bernard Timothy

AU - Krupski, W. C.

AU - Tilson, M. D.

AU - Clowes, A. W.

AU - Nolan, K.

AU - Hunt, T. K.

AU - Cohen, J.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Background. Although increased procollagen gene expression and synthesis have been implicated in the progression of abdominal aortic aneurysms (AAA), factors modulating this change have not been identified. Furthermore, it is not known whether the increase in AAA procollagen expression is specific to this disease or also occurs in tissue affected by atherosclerotic occlusive disease (AOD). If paracrine rather than autocrine factors are responsible for increased gene expression in AAA, this effect should be transferable to target smooth muscle cells through conditioned media. Our objectives were to determine 1α(I) procollagen messenger RNA levels in AOD tissue compared with normal and AAA and to determine whether differences noted in tissue procollagen gene expression could be transferred through conditioned media from normal, AOD, and AAA tissues to target smooth muscle cells in primary culture. Methods. Normal, AOD, and AAA tissue was used for tissue RNA extraction or was minced and washed with serum-free media (4° C) X 30 minutes and the media applied to human aortic smooth muscle cells (SMC) in primary culture for 36 hours. Total RNA from tissue and SMC exposed to conditioned media was analyzed by Northern and dot blot analysis for 1α(I) procollagen. Results. Relative tissue 1α(I) procollagen levels were not increased in AOD (0.23 ± 0.05) as compared with normal (0.17 ± 0.03); both were decreased compared with AAA (0.53 ± 0.07; p < 0.01). The 1α(I) procollagen levels in SMC exposed to conditioned media from AAA (1.73 ± 0.15) were increased (p < 0.05) compared with AOD (1.10 ± 0.12) and normal (1.16 ± 0.16). Conclusions. There is no increase in tissue AOD procollagen gene expression. The ability to transfer the same relative patterns of gene expression from tissue to target SMC with conditioned media suggests that paracrine, rather than autocrine, factors modulate procollagen expression in AAA tissues.

AB - Background. Although increased procollagen gene expression and synthesis have been implicated in the progression of abdominal aortic aneurysms (AAA), factors modulating this change have not been identified. Furthermore, it is not known whether the increase in AAA procollagen expression is specific to this disease or also occurs in tissue affected by atherosclerotic occlusive disease (AOD). If paracrine rather than autocrine factors are responsible for increased gene expression in AAA, this effect should be transferable to target smooth muscle cells through conditioned media. Our objectives were to determine 1α(I) procollagen messenger RNA levels in AOD tissue compared with normal and AAA and to determine whether differences noted in tissue procollagen gene expression could be transferred through conditioned media from normal, AOD, and AAA tissues to target smooth muscle cells in primary culture. Methods. Normal, AOD, and AAA tissue was used for tissue RNA extraction or was minced and washed with serum-free media (4° C) X 30 minutes and the media applied to human aortic smooth muscle cells (SMC) in primary culture for 36 hours. Total RNA from tissue and SMC exposed to conditioned media was analyzed by Northern and dot blot analysis for 1α(I) procollagen. Results. Relative tissue 1α(I) procollagen levels were not increased in AOD (0.23 ± 0.05) as compared with normal (0.17 ± 0.03); both were decreased compared with AAA (0.53 ± 0.07; p < 0.01). The 1α(I) procollagen levels in SMC exposed to conditioned media from AAA (1.73 ± 0.15) were increased (p < 0.05) compared with AOD (1.10 ± 0.12) and normal (1.16 ± 0.16). Conclusions. There is no increase in tissue AOD procollagen gene expression. The ability to transfer the same relative patterns of gene expression from tissue to target SMC with conditioned media suggests that paracrine, rather than autocrine, factors modulate procollagen expression in AAA tissues.

UR - http://www.scopus.com/inward/record.url?scp=0027235719&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027235719&partnerID=8YFLogxK

M3 - Article

C2 - 8342129

AN - SCOPUS:0027235719

VL - 114

SP - 252

EP - 257

JO - Surgery (United States)

JF - Surgery (United States)

SN - 0039-6060

IS - 2

ER -