Solid‐phase peptide synthesis and biological activity of bovine thymopoietin II (bTP–11)

D. D. Smith, J. M. Conlon, J. Petzel, L. Chen, R. F. Murphy, Barbara J Morley

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Bovine thymopoietin (bTP), a 49 amino acid polypeptide, was synthesized using Merrifield's solid‐phase peptide synthesis methodology. The polypeptide was purified using anion‐exchange chromatography and reversed‐phase HPLC and characterized by mass spectrometry and amino acid analysis of the full‐length peptide and of products derived from digestion with Staphylococcus aureus V8 protease. The biological activity of the synthesized product was tested in several assay systems. Synthetic bTP was found to induce the expression of Thy 1.2 antigen on T‐lymphocytes from athymic mice, in agreement with previous studies on the biological activity of endogenous bTP. Biological activity at skeletal muscle and neuronal nicotinic acetylcholine receptor sites, as reported by others for bTP, could not be confirmed in our studies. The absence of biological activity at nicotinic receptor sites may be related to the results of a recent report demonstrating the presence of a cobratoxin‐like molecule in preparations of natural bTP. These data indicate that synthetic peptides have an important role for the evaluation of the specificity of the biological activity of polypeptides.

Original languageEnglish (US)
Pages (from-to)183-191
Number of pages9
JournalInternational Journal of Peptide and Protein Research
Volume44
Issue number2
DOIs
StatePublished - Aug 1994

Fingerprint

Thymopoietins
Bioactivity
Peptides
Nicotinic Receptors
Thy-1 Antigens
Amino Acids
Chromatography
Nude Mice
Mass spectrometry
Muscle
Staphylococcus aureus
Digestion
Assays
Mass Spectrometry
Skeletal Muscle
High Pressure Liquid Chromatography
Molecules

Keywords

  • bovine thymopoietin II
  • nicotinic acetylcholine receptors
  • peptide mapping
  • solid‐phase peptide synthesis
  • thymic peptides
  • β‐aspartyl shift

ASJC Scopus subject areas

  • Biochemistry

Cite this

Solid‐phase peptide synthesis and biological activity of bovine thymopoietin II (bTP–11). / Smith, D. D.; Conlon, J. M.; Petzel, J.; Chen, L.; Murphy, R. F.; Morley, Barbara J.

In: International Journal of Peptide and Protein Research, Vol. 44, No. 2, 08.1994, p. 183-191.

Research output: Contribution to journalArticle

Smith, D. D. ; Conlon, J. M. ; Petzel, J. ; Chen, L. ; Murphy, R. F. ; Morley, Barbara J. / Solid‐phase peptide synthesis and biological activity of bovine thymopoietin II (bTP–11). In: International Journal of Peptide and Protein Research. 1994 ; Vol. 44, No. 2. pp. 183-191.
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