Solid-phase combinatorial synthesis using MicroKan reactors, Rf tagging, and directed sorting

Xiao Yi Xiao, Rongshi Li, Hui Zhuang, Bill Ewing, K. Karunaratne, John Lillig, Rick Brown, K. C. Nicolaou

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A modular system for high-output solid-phase combinatorial synthesis has been designed and developed. The system employs three technological innovations to achieve its high efficiency and reliability: (1) application of microreactors as the reaction units in solid-phase synthesis; (2) use of radiofrequency tagging as the non-chemical tracking method; and (3) development of the directed sorting technology for split and pool synthesis. The system has been successfully applied in the synthesis of compound libraries of several hundred to several thousand compounds in multi- milligrams per compound quantity by many organizations. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalBiotechnology and Bioengineering
Volume71
Issue number1
DOIs
StatePublished - Dec 1 2000
Externally publishedYes

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Solid-Phase Synthesis Techniques
Sorting
Inventions
Libraries
Technology
Innovation

Keywords

  • Directed sorting
  • MicroKan reactor
  • Parallel synthesis
  • Radiofrequency tagging
  • Solid-phase synthesis
  • Split and pool

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Solid-phase combinatorial synthesis using MicroKan reactors, Rf tagging, and directed sorting. / Xiao, Xiao Yi; Li, Rongshi; Zhuang, Hui; Ewing, Bill; Karunaratne, K.; Lillig, John; Brown, Rick; Nicolaou, K. C.

In: Biotechnology and Bioengineering, Vol. 71, No. 1, 01.12.2000, p. 44-50.

Research output: Contribution to journalArticle

Xiao, Xiao Yi ; Li, Rongshi ; Zhuang, Hui ; Ewing, Bill ; Karunaratne, K. ; Lillig, John ; Brown, Rick ; Nicolaou, K. C. / Solid-phase combinatorial synthesis using MicroKan reactors, Rf tagging, and directed sorting. In: Biotechnology and Bioengineering. 2000 ; Vol. 71, No. 1. pp. 44-50.
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