Soft agar growth assays for transforming growth factors and mitogenic peptides

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Many different tumor cells produce and release transforming growth factors (TGFs) that influence the growth and behavior of the cells that produce them. Consequently, the production of TGFs may have important effects on the growth of tumors in vivo. The ability to grow in soft agar is one of the best indicators of a cell's tumorigenic capacity. TGFs can induce the soft agar growth of nontransformed cells. TGFs are usually assayed by their ability to induce the soft agar growth of nontransformed cells. This chapter describes methods to examine TGFs and related growth factors for their ability to influence anchorage-independent growth. The chapter discusses the most commonly used indicator cell lines, the general methodology employed, the selection of appropriate culture media, and a rapid assay that is suitable for testing small numbers of cells for the release of factors that influence anchorage-independent growth.

Original languageEnglish (US)
Pages (from-to)341-352
Number of pages12
JournalMethods in enzymology
Volume146
Issue numberC
DOIs
StatePublished - Jan 1 1987

Fingerprint

Transforming Growth Factors
Agar
Assays
Peptides
Cells
Growth
Tumors
Culture Media
Neoplasms
Intercellular Signaling Peptides and Proteins
Cell Count
Cell Line
Testing

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Soft agar growth assays for transforming growth factors and mitogenic peptides. / Rizzino, A Angie.

In: Methods in enzymology, Vol. 146, No. C, 01.01.1987, p. 341-352.

Research output: Contribution to journalArticle

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