Sodium‐Dependent Uptake of Nucleosides by Dissociated Brain Cells from the Rat

Mark E. Johnston, Jonathan D. Geiger

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Abstract: Sodium‐dependent 3H‐labeled nucleoside transport was studied using a mixed population of dissociated brain cells from adult rats. The accumulation of [3H]adenosine during brief (15‐s) incubation periods was significantly greater in the presence of 110 μM Na+ than in its absence. This occurred at substrate concentrations that ranged from 0.25 to 100 μM. Similar findings were observed for the rapid accumulation of [3H]uridine. Kinetically, the rapid accumulation of [3H]adenosine in both the absence and the presence of Na+ was best described by a two‐component system. In the presence of Na+, the KT and Vmax values for the high‐affinity component were 0.9 μM and 8.9 pmol/mg of protein/15 s, and those for the low‐affinity component were 313 μM and 3,428 pmol/mg of protein/15 s, respectively. In the absence of Na+, the KT value for the high‐affinity component was significantly higher (1.8 μM). [3H]Uridine accumulation was best described kinetically by a one‐component system that in the presence of Na+ had KT and Vmax values of 1.0 mM and 2.6 nmol/mg of protein/15 s, respectively. As was found for [3H]adenosine, in the absence of Na+, the KT value was significantly higher (1.8 mM). The sodium‐dependent transport of [3H]adenosine was inhibitable by ouabain and 2,4‐dinitrophenol. Of the three nucleoside transport inhibitors tested, only nitrobenzylthioinosine demonstrated high affinity and selectivity in blocking the sodium component. Thus, high‐affinity sodium‐dependent nucleoside transport systems, in addition to facilitated diffusion systems, exist on brain cells from adult rats.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalJournal of Neurochemistry
Volume52
Issue number1
DOIs
StatePublished - Jan 1989

Fingerprint

Nucleosides
Adenosine
Rats
Brain
Uridine
Facilitated Diffusion
Proteins
Ouabain
Sodium
Substrates
Population

Keywords

  • Adenosine
  • Brain cells
  • Nucleosides
  • Sodium dependency
  • Transport
  • Uptake
  • Uridine

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Sodium‐Dependent Uptake of Nucleosides by Dissociated Brain Cells from the Rat. / Johnston, Mark E.; Geiger, Jonathan D.

In: Journal of Neurochemistry, Vol. 52, No. 1, 01.1989, p. 75-81.

Research output: Contribution to journalArticle

@article{08dc2a5dd5b94e569f6e17ebb71ffc24,
title = "Sodium‐Dependent Uptake of Nucleosides by Dissociated Brain Cells from the Rat",
abstract = "Abstract: Sodium‐dependent 3H‐labeled nucleoside transport was studied using a mixed population of dissociated brain cells from adult rats. The accumulation of [3H]adenosine during brief (15‐s) incubation periods was significantly greater in the presence of 110 μM Na+ than in its absence. This occurred at substrate concentrations that ranged from 0.25 to 100 μM. Similar findings were observed for the rapid accumulation of [3H]uridine. Kinetically, the rapid accumulation of [3H]adenosine in both the absence and the presence of Na+ was best described by a two‐component system. In the presence of Na+, the KT and Vmax values for the high‐affinity component were 0.9 μM and 8.9 pmol/mg of protein/15 s, and those for the low‐affinity component were 313 μM and 3,428 pmol/mg of protein/15 s, respectively. In the absence of Na+, the KT value for the high‐affinity component was significantly higher (1.8 μM). [3H]Uridine accumulation was best described kinetically by a one‐component system that in the presence of Na+ had KT and Vmax values of 1.0 mM and 2.6 nmol/mg of protein/15 s, respectively. As was found for [3H]adenosine, in the absence of Na+, the KT value was significantly higher (1.8 mM). The sodium‐dependent transport of [3H]adenosine was inhibitable by ouabain and 2,4‐dinitrophenol. Of the three nucleoside transport inhibitors tested, only nitrobenzylthioinosine demonstrated high affinity and selectivity in blocking the sodium component. Thus, high‐affinity sodium‐dependent nucleoside transport systems, in addition to facilitated diffusion systems, exist on brain cells from adult rats.",
keywords = "Adenosine, Brain cells, Nucleosides, Sodium dependency, Transport, Uptake, Uridine",
author = "Johnston, {Mark E.} and Geiger, {Jonathan D.}",
year = "1989",
month = "1",
doi = "10.1111/j.1471-4159.1989.tb10900.x",
language = "English (US)",
volume = "52",
pages = "75--81",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Sodium‐Dependent Uptake of Nucleosides by Dissociated Brain Cells from the Rat

AU - Johnston, Mark E.

AU - Geiger, Jonathan D.

PY - 1989/1

Y1 - 1989/1

N2 - Abstract: Sodium‐dependent 3H‐labeled nucleoside transport was studied using a mixed population of dissociated brain cells from adult rats. The accumulation of [3H]adenosine during brief (15‐s) incubation periods was significantly greater in the presence of 110 μM Na+ than in its absence. This occurred at substrate concentrations that ranged from 0.25 to 100 μM. Similar findings were observed for the rapid accumulation of [3H]uridine. Kinetically, the rapid accumulation of [3H]adenosine in both the absence and the presence of Na+ was best described by a two‐component system. In the presence of Na+, the KT and Vmax values for the high‐affinity component were 0.9 μM and 8.9 pmol/mg of protein/15 s, and those for the low‐affinity component were 313 μM and 3,428 pmol/mg of protein/15 s, respectively. In the absence of Na+, the KT value for the high‐affinity component was significantly higher (1.8 μM). [3H]Uridine accumulation was best described kinetically by a one‐component system that in the presence of Na+ had KT and Vmax values of 1.0 mM and 2.6 nmol/mg of protein/15 s, respectively. As was found for [3H]adenosine, in the absence of Na+, the KT value was significantly higher (1.8 mM). The sodium‐dependent transport of [3H]adenosine was inhibitable by ouabain and 2,4‐dinitrophenol. Of the three nucleoside transport inhibitors tested, only nitrobenzylthioinosine demonstrated high affinity and selectivity in blocking the sodium component. Thus, high‐affinity sodium‐dependent nucleoside transport systems, in addition to facilitated diffusion systems, exist on brain cells from adult rats.

AB - Abstract: Sodium‐dependent 3H‐labeled nucleoside transport was studied using a mixed population of dissociated brain cells from adult rats. The accumulation of [3H]adenosine during brief (15‐s) incubation periods was significantly greater in the presence of 110 μM Na+ than in its absence. This occurred at substrate concentrations that ranged from 0.25 to 100 μM. Similar findings were observed for the rapid accumulation of [3H]uridine. Kinetically, the rapid accumulation of [3H]adenosine in both the absence and the presence of Na+ was best described by a two‐component system. In the presence of Na+, the KT and Vmax values for the high‐affinity component were 0.9 μM and 8.9 pmol/mg of protein/15 s, and those for the low‐affinity component were 313 μM and 3,428 pmol/mg of protein/15 s, respectively. In the absence of Na+, the KT value for the high‐affinity component was significantly higher (1.8 μM). [3H]Uridine accumulation was best described kinetically by a one‐component system that in the presence of Na+ had KT and Vmax values of 1.0 mM and 2.6 nmol/mg of protein/15 s, respectively. As was found for [3H]adenosine, in the absence of Na+, the KT value was significantly higher (1.8 mM). The sodium‐dependent transport of [3H]adenosine was inhibitable by ouabain and 2,4‐dinitrophenol. Of the three nucleoside transport inhibitors tested, only nitrobenzylthioinosine demonstrated high affinity and selectivity in blocking the sodium component. Thus, high‐affinity sodium‐dependent nucleoside transport systems, in addition to facilitated diffusion systems, exist on brain cells from adult rats.

KW - Adenosine

KW - Brain cells

KW - Nucleosides

KW - Sodium dependency

KW - Transport

KW - Uptake

KW - Uridine

UR - http://www.scopus.com/inward/record.url?scp=0024508296&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024508296&partnerID=8YFLogxK

U2 - 10.1111/j.1471-4159.1989.tb10900.x

DO - 10.1111/j.1471-4159.1989.tb10900.x

M3 - Article

C2 - 2908894

AN - SCOPUS:0024508296

VL - 52

SP - 75

EP - 81

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 1

ER -