Sodium nitroprusside increases glomerular capillary hydraulic conductivity in isolated rat glomeruli.

Helen Bergado Lovell, R. Sharma, J. Z. Li, V. J. Savin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Sodium nitroprusside (SNP) causes renal vasodilation, increased renal blood flow, and increased renal sodium excretion. Systemic vascular effects of SNP are dependent on generation of nitric oxide and increase in smooth muscle intracellular cyclic guanosine monophosphate (cGMP). In the current studies isolated glomeruli were used to determine the effects of SNP on glomerular capillary hydraulic conductivity (Lp) and on mesangial tone. The direct effects of the cGMP analogue 8-bromo-cGMP on Lp were studied. Glomeruli were isolated from the superficial renal cortex of adult male Sprague-Dawley rats weighing 143 to 383 gm. Lp was calculated from videomicroscopic images of individual glomeruli during the initial 0.1 second of filtration induced by an albumin oncotic gradient. Incubation of glomeruli in 10(-3) mol/L SNP increased Lp from a control value of 1.38 +/- 0.08 microliter/min.mm Hg.cm2 to 1.91 +/- 0.13 microliter.min.mm Hg.cm2 (p < 0.01). Incubation of glomeruli in 10(-5) mol/L 8-bromo-cGMP increased Lp to a comparable degree from a control value of 2.00 +/- 0.58 microliter/.min.mm Hg.cm2 to 2.39 +/- 0.62 microliter/min.mm Hg.cm2 (p < 0.03). Increase in Lp was observed independent of any effects on systemic or renal circulations, neural effects, or humoral effects. Changes in mesangial tone were estimated from changes in glomerular volume during 15 minutes of incubation with SNP or control medium. Incubation of glomeruli in 10(-5) mol/L SNP increased glomerular volume 6%, a consequence of mesangial relaxation. Incubation of glomeruli in 10(-7) and 10(-3) mol/L SNP did not affect mesangial tone.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)450-455
Number of pages6
JournalJournal of Laboratory and Clinical Medicine
Volume125
Issue number4
StatePublished - Apr 1 1995
Externally publishedYes

Fingerprint

Hydraulic conductivity
Nitroprusside
Rats
Cyclic GMP
Renal Circulation
Kidney
Weighing
Vasodilation
Smooth Muscle
Blood Vessels
Sprague Dawley Rats
Muscle
Albumins
Nitric Oxide
Blood
Sodium

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Sodium nitroprusside increases glomerular capillary hydraulic conductivity in isolated rat glomeruli. / Lovell, Helen Bergado; Sharma, R.; Li, J. Z.; Savin, V. J.

In: Journal of Laboratory and Clinical Medicine, Vol. 125, No. 4, 01.04.1995, p. 450-455.

Research output: Contribution to journalArticle

@article{10fc0c5a28d8470cb861e6930bb5a8f3,
title = "Sodium nitroprusside increases glomerular capillary hydraulic conductivity in isolated rat glomeruli.",
abstract = "Sodium nitroprusside (SNP) causes renal vasodilation, increased renal blood flow, and increased renal sodium excretion. Systemic vascular effects of SNP are dependent on generation of nitric oxide and increase in smooth muscle intracellular cyclic guanosine monophosphate (cGMP). In the current studies isolated glomeruli were used to determine the effects of SNP on glomerular capillary hydraulic conductivity (Lp) and on mesangial tone. The direct effects of the cGMP analogue 8-bromo-cGMP on Lp were studied. Glomeruli were isolated from the superficial renal cortex of adult male Sprague-Dawley rats weighing 143 to 383 gm. Lp was calculated from videomicroscopic images of individual glomeruli during the initial 0.1 second of filtration induced by an albumin oncotic gradient. Incubation of glomeruli in 10(-3) mol/L SNP increased Lp from a control value of 1.38 +/- 0.08 microliter/min.mm Hg.cm2 to 1.91 +/- 0.13 microliter.min.mm Hg.cm2 (p < 0.01). Incubation of glomeruli in 10(-5) mol/L 8-bromo-cGMP increased Lp to a comparable degree from a control value of 2.00 +/- 0.58 microliter/.min.mm Hg.cm2 to 2.39 +/- 0.62 microliter/min.mm Hg.cm2 (p < 0.03). Increase in Lp was observed independent of any effects on systemic or renal circulations, neural effects, or humoral effects. Changes in mesangial tone were estimated from changes in glomerular volume during 15 minutes of incubation with SNP or control medium. Incubation of glomeruli in 10(-5) mol/L SNP increased glomerular volume 6{\%}, a consequence of mesangial relaxation. Incubation of glomeruli in 10(-7) and 10(-3) mol/L SNP did not affect mesangial tone.(ABSTRACT TRUNCATED AT 250 WORDS)",
author = "Lovell, {Helen Bergado} and R. Sharma and Li, {J. Z.} and Savin, {V. J.}",
year = "1995",
month = "4",
day = "1",
language = "English (US)",
volume = "125",
pages = "450--455",
journal = "Translational Research",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "4",

}

TY - JOUR

T1 - Sodium nitroprusside increases glomerular capillary hydraulic conductivity in isolated rat glomeruli.

AU - Lovell, Helen Bergado

AU - Sharma, R.

AU - Li, J. Z.

AU - Savin, V. J.

PY - 1995/4/1

Y1 - 1995/4/1

N2 - Sodium nitroprusside (SNP) causes renal vasodilation, increased renal blood flow, and increased renal sodium excretion. Systemic vascular effects of SNP are dependent on generation of nitric oxide and increase in smooth muscle intracellular cyclic guanosine monophosphate (cGMP). In the current studies isolated glomeruli were used to determine the effects of SNP on glomerular capillary hydraulic conductivity (Lp) and on mesangial tone. The direct effects of the cGMP analogue 8-bromo-cGMP on Lp were studied. Glomeruli were isolated from the superficial renal cortex of adult male Sprague-Dawley rats weighing 143 to 383 gm. Lp was calculated from videomicroscopic images of individual glomeruli during the initial 0.1 second of filtration induced by an albumin oncotic gradient. Incubation of glomeruli in 10(-3) mol/L SNP increased Lp from a control value of 1.38 +/- 0.08 microliter/min.mm Hg.cm2 to 1.91 +/- 0.13 microliter.min.mm Hg.cm2 (p < 0.01). Incubation of glomeruli in 10(-5) mol/L 8-bromo-cGMP increased Lp to a comparable degree from a control value of 2.00 +/- 0.58 microliter/.min.mm Hg.cm2 to 2.39 +/- 0.62 microliter/min.mm Hg.cm2 (p < 0.03). Increase in Lp was observed independent of any effects on systemic or renal circulations, neural effects, or humoral effects. Changes in mesangial tone were estimated from changes in glomerular volume during 15 minutes of incubation with SNP or control medium. Incubation of glomeruli in 10(-5) mol/L SNP increased glomerular volume 6%, a consequence of mesangial relaxation. Incubation of glomeruli in 10(-7) and 10(-3) mol/L SNP did not affect mesangial tone.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - Sodium nitroprusside (SNP) causes renal vasodilation, increased renal blood flow, and increased renal sodium excretion. Systemic vascular effects of SNP are dependent on generation of nitric oxide and increase in smooth muscle intracellular cyclic guanosine monophosphate (cGMP). In the current studies isolated glomeruli were used to determine the effects of SNP on glomerular capillary hydraulic conductivity (Lp) and on mesangial tone. The direct effects of the cGMP analogue 8-bromo-cGMP on Lp were studied. Glomeruli were isolated from the superficial renal cortex of adult male Sprague-Dawley rats weighing 143 to 383 gm. Lp was calculated from videomicroscopic images of individual glomeruli during the initial 0.1 second of filtration induced by an albumin oncotic gradient. Incubation of glomeruli in 10(-3) mol/L SNP increased Lp from a control value of 1.38 +/- 0.08 microliter/min.mm Hg.cm2 to 1.91 +/- 0.13 microliter.min.mm Hg.cm2 (p < 0.01). Incubation of glomeruli in 10(-5) mol/L 8-bromo-cGMP increased Lp to a comparable degree from a control value of 2.00 +/- 0.58 microliter/.min.mm Hg.cm2 to 2.39 +/- 0.62 microliter/min.mm Hg.cm2 (p < 0.03). Increase in Lp was observed independent of any effects on systemic or renal circulations, neural effects, or humoral effects. Changes in mesangial tone were estimated from changes in glomerular volume during 15 minutes of incubation with SNP or control medium. Incubation of glomeruli in 10(-5) mol/L SNP increased glomerular volume 6%, a consequence of mesangial relaxation. Incubation of glomeruli in 10(-7) and 10(-3) mol/L SNP did not affect mesangial tone.(ABSTRACT TRUNCATED AT 250 WORDS)

UR - http://www.scopus.com/inward/record.url?scp=0029282233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029282233&partnerID=8YFLogxK

M3 - Article

VL - 125

SP - 450

EP - 455

JO - Translational Research

JF - Translational Research

SN - 1931-5244

IS - 4

ER -