Sodium dodecyl sulfate hypersensitivity of clpP and clpB mutants of Escherichia coli

Soumitra Rajagopal, Narasimhan Sudarsan, Kenneth Nickerson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We studied the hypersensitivity of clpP and clpB mutants of Escherichia coli to sodium dodecyl sulfate (SDS). Both wild-type E. coli MC4100 and Ion mutants grew in the presence of 10% SDS, whereas isogenic clpP and clpB single mutants could not grow above 0.5% SDS and clpA and clpX single mutants could not grow above 5.0% SDS. For wild-type E. coli, cellular ClpP levels as determined by Western immunoblot analysis increased ca. sixfold as the levels of added SDS increased from 0 to 2%. Capsular colanic acid, measured as uronic acid, increased ca. sixfold as the levels of added SDS increased from 2 to 10%. Based on these findings, 3 of the 19 previously identified SDS shock proteins (M. Adamowicz, P. M. Kelley, and K. W. Nickerson, J. Bacteriol. 173:229-233, 1991) are tentatively identified as ClpP, ClpX, and ClpB.

Original languageEnglish (US)
Pages (from-to)4117-4121
Number of pages5
JournalApplied and environmental microbiology
Volume68
Issue number8
DOIs
StatePublished - Aug 13 2002

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sodium dodecyl sulfate
hypersensitivity
Sodium Dodecyl Sulfate
Hypersensitivity
sodium
Escherichia coli
sulfate
mutants
Uronic Acids
uronic acids
acid
mutant
Shock
Western Blotting
Ions
ions
protein
ion
acids
Proteins

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Sodium dodecyl sulfate hypersensitivity of clpP and clpB mutants of Escherichia coli. / Rajagopal, Soumitra; Sudarsan, Narasimhan; Nickerson, Kenneth.

In: Applied and environmental microbiology, Vol. 68, No. 8, 13.08.2002, p. 4117-4121.

Research output: Contribution to journalArticle

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