Sodium-activated potassium channels shape peripheral auditory function and activity of the primary auditory neurons in mice

Daniël O.J. Reijntjes, Jeong Han Lee, Seojin Park, Nick M.A. Schubert, Marcel van Tuinen, Sarath Vijayakumar, Timothy A. Jones, Sherri M. Jones, Michael Anne Gratton, Xiao Ming Xia, Ebenezer N. Yamoah, Sonja J. Pyott

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Abstract

Potassium (K + ) channels shape the response properties of neurons. Although enormous progress has been made to characterize K + channels in the primary auditory neurons, the molecular identities of many of these channels and their contributions to hearing in vivo remain unknown. Using a combination of RNA sequencing and single molecule fluorescent in situ hybridization, we localized expression of transcripts encoding the sodium-activated potassium channels K Na 1.1 (SLO2.2/Slack) and K Na 1.2 (SLO2.1/Slick) to the primary auditory neurons (spiral ganglion neurons, SGNs). To examine the contribution of these channels to function of the SGNs in vivo, we measured auditory brainstem responses in K Na 1.1/1.2 double knockout (DKO) mice. Although auditory brainstem response (wave I) thresholds were not altered, the amplitudes of suprathreshold responses were reduced in DKO mice. This reduction in amplitude occurred despite normal numbers and molecular architecture of the SGNs and their synapses with the inner hair cells. Patch clamp electrophysiology of SGNs isolated from DKO mice displayed altered membrane properties, including reduced action potential thresholds and amplitudes. These findings show that K Na 1 channel activity is essential for normal cochlear function and suggest that early forms of hearing loss may result from physiological changes in the activity of the primary auditory neurons.

Original languageEnglish (US)
Article number2573
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Potassium Channels
Sodium
Spiral Ganglion
Neurons
Knockout Mice
Brain Stem Auditory Evoked Potentials
Inner Auditory Hair Cells
RNA Sequence Analysis
Electrophysiology
Cochlea
Fluorescence In Situ Hybridization
Hearing Loss
Synapses
Hearing
Action Potentials
Membranes

ASJC Scopus subject areas

  • General

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Sodium-activated potassium channels shape peripheral auditory function and activity of the primary auditory neurons in mice. / Reijntjes, Daniël O.J.; Lee, Jeong Han; Park, Seojin; Schubert, Nick M.A.; van Tuinen, Marcel; Vijayakumar, Sarath; Jones, Timothy A.; Jones, Sherri M.; Gratton, Michael Anne; Xia, Xiao Ming; Yamoah, Ebenezer N.; Pyott, Sonja J.

In: Scientific reports, Vol. 9, No. 1, 2573, 01.12.2019.

Research output: Contribution to journalArticle

Reijntjes, Daniël O.J. ; Lee, Jeong Han ; Park, Seojin ; Schubert, Nick M.A. ; van Tuinen, Marcel ; Vijayakumar, Sarath ; Jones, Timothy A. ; Jones, Sherri M. ; Gratton, Michael Anne ; Xia, Xiao Ming ; Yamoah, Ebenezer N. ; Pyott, Sonja J. / Sodium-activated potassium channels shape peripheral auditory function and activity of the primary auditory neurons in mice. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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