snRNA 3′ End Processing by a CPSF73-Containing Complex Essential for Development in Arabidopsis

Yunfeng Liu, Shengjun Li, Yuan Chen, Athen N. Kimberlin, Edgar B. Cahoon, Bin Yu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Uridine-rich small nuclear RNAs (snRNAs) are the basal components of the spliceosome and play essential roles in splicing. The biogenesis of the majority of snRNAs involves 3′ end endonucleolytic cleavage of the nascent transcript from the elongating DNA-dependent RNA ploymerase II. However, the protein factors responsible for this process remain elusive in plants. Here, we show that DEFECTIVE in snRNA PROCESSING 1 (DSP1) is an essential protein for snRNA 3′ end maturation in Arabidopsis. A hypomorphic dsp1-1 mutation causes pleiotropic developmental defects, impairs the 3′ end processing of snRNAs, increases the levels of snRNA primary transcripts (pre-snRNAs), and alters the occupancy of Pol II at snRNA loci. In addition, DSP1 binds snRNA loci and interacts with Pol-II in a DNA/RNA-dependent manner. We further show that DSP1 forms a conserved complex, which contains at least four additional proteins, to catalyze snRNA 3′ end maturation in Arabidopsis. The catalytic component of this complex is likely the cleavage and polyadenylation specificity factor 73 kDa-I (CSPF73-I), which is the nuclease cleaving the pre-mRNA 3′ end. However, the DSP1 complex does not affect pre-mRNA 3′ end cleavage, suggesting that plants may use different CPSF73-I-containing complexes to process snRNAs and pre-mRNAs. This study identifies a complex responsible for the snRNA 3′ end maturation in plants and uncovers a previously unknown function of CPSF73 in snRNA maturation.

Original languageEnglish (US)
Article numbere1002571
JournalPLoS biology
Volume14
Issue number10
DOIs
StatePublished - Oct 25 2016

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small nuclear RNA
Small Nuclear RNA
Arabidopsis
Processing
RNA Precursors
Cleavage And Polyadenylation Specificity Factor
spliceosomes
RNA
Spliceosomes
loci
Proteins
uridine
proteins
nucleases

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

snRNA 3′ End Processing by a CPSF73-Containing Complex Essential for Development in Arabidopsis. / Liu, Yunfeng; Li, Shengjun; Chen, Yuan; Kimberlin, Athen N.; Cahoon, Edgar B.; Yu, Bin.

In: PLoS biology, Vol. 14, No. 10, e1002571, 25.10.2016.

Research output: Contribution to journalArticle

Liu, Yunfeng ; Li, Shengjun ; Chen, Yuan ; Kimberlin, Athen N. ; Cahoon, Edgar B. ; Yu, Bin. / snRNA 3′ End Processing by a CPSF73-Containing Complex Essential for Development in Arabidopsis. In: PLoS biology. 2016 ; Vol. 14, No. 10.
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