Smokeless tobacco effects on monocyte secretion of PGE2 and IL-1 beta.

Jeffrey B Payne, G. K. Johnson, Richard A Reinhardt, C. R. Maze, J. K. Dyer, K. D. Patil

Research output: Contribution to journalArticle

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Abstract

The use of smokeless tobacco (ST) products is associated with mucosal lesions, gingival recession, and attachment loss at the site of tobacco placement. Monocytes/macrophages are primary producers of PGE2 and IL-1 beta, inflammatory mediators which are thought to play a role in the destruction of the periodontium. The purpose of this study was to determine the effect of ST alone and in combination with a major stimulator of inflammation, bacterial lipopolysaccharide (LPS), on monocyte secretion of these mediators. Peripheral blood monocytes (PBM) were isolated by counterflow centrifugal elutriation from 15 healthy donors who were non-ST users. PBM were incubated for 24 hours in RPMI 1640 containing various concentrations of ST (0%, 0.005%, 0.01%, 1%) with or without 10 micrograms/ml LPS (Porphyromonas gingivalis LPS or Escherichia coli LPS). Of the ST preparations, only 1% ST resulted in PBM mediator secretion (7.7 +/- 2.0 ng/ml for PGE2 and 1.3 +/- 0.2 ng/ml for IL-1 beta) above that of control (unstimulated) cultures. Furthermore, the combination of 1% ST and LPS resulted in a potentiation of PGE2 release (5-fold for E. coli LPS + 1% ST and 10-fold for P. gingivalis LPS + 1% ST; P < 0.0001, one-way ANOVA) relative to the LPS preparations alone. In contrast, PBM IL-1 beta release decreased more than 2-fold upon E. coli LPS and 1% ST exposure, relative to treatment with E. coli LPS alone (P < 0.0001, one-way ANOVA).(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)937-941
Number of pages5
JournalJournal of periodontology
Volume65
Issue number10
DOIs
StatePublished - Oct 1994

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Smokeless Tobacco
Interleukin-1beta
Dinoprostone
Lipopolysaccharides
Monocytes
Escherichia coli
Porphyromonas gingivalis
Tobacco
Analysis of Variance
Gingival Recession
Periodontium
Tobacco Products
Macrophages
Inflammation

ASJC Scopus subject areas

  • Periodontics

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Smokeless tobacco effects on monocyte secretion of PGE2 and IL-1 beta. / Payne, Jeffrey B; Johnson, G. K.; Reinhardt, Richard A; Maze, C. R.; Dyer, J. K.; Patil, K. D.

In: Journal of periodontology, Vol. 65, No. 10, 10.1994, p. 937-941.

Research output: Contribution to journalArticle

Payne, Jeffrey B ; Johnson, G. K. ; Reinhardt, Richard A ; Maze, C. R. ; Dyer, J. K. ; Patil, K. D. / Smokeless tobacco effects on monocyte secretion of PGE2 and IL-1 beta. In: Journal of periodontology. 1994 ; Vol. 65, No. 10. pp. 937-941.
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abstract = "The use of smokeless tobacco (ST) products is associated with mucosal lesions, gingival recession, and attachment loss at the site of tobacco placement. Monocytes/macrophages are primary producers of PGE2 and IL-1 beta, inflammatory mediators which are thought to play a role in the destruction of the periodontium. The purpose of this study was to determine the effect of ST alone and in combination with a major stimulator of inflammation, bacterial lipopolysaccharide (LPS), on monocyte secretion of these mediators. Peripheral blood monocytes (PBM) were isolated by counterflow centrifugal elutriation from 15 healthy donors who were non-ST users. PBM were incubated for 24 hours in RPMI 1640 containing various concentrations of ST (0{\%}, 0.005{\%}, 0.01{\%}, 1{\%}) with or without 10 micrograms/ml LPS (Porphyromonas gingivalis LPS or Escherichia coli LPS). Of the ST preparations, only 1{\%} ST resulted in PBM mediator secretion (7.7 +/- 2.0 ng/ml for PGE2 and 1.3 +/- 0.2 ng/ml for IL-1 beta) above that of control (unstimulated) cultures. Furthermore, the combination of 1{\%} ST and LPS resulted in a potentiation of PGE2 release (5-fold for E. coli LPS + 1{\%} ST and 10-fold for P. gingivalis LPS + 1{\%} ST; P < 0.0001, one-way ANOVA) relative to the LPS preparations alone. In contrast, PBM IL-1 beta release decreased more than 2-fold upon E. coli LPS and 1{\%} ST exposure, relative to treatment with E. coli LPS alone (P < 0.0001, one-way ANOVA).(ABSTRACT TRUNCATED AT 250 WORDS)",
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