Smad3 mediates TGF-β1 induction of VEGF production in lung fibroblasts

Tetsu Kobayashi, Xiang-de Liu, Fu Qiang Wen, Qiuhong Fang, Shinji Abe, Xiang Qi Wang, Mitsuyoshi Hashimoto, Lei Shen, Shin Kawasaki, Hui Jung Kim, Tadashi Kohyama, Stephen I. Rennard

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Transforming growth factor-β1 (TGF-β1) is a key factor in a variety of physiological and pathological processes. Vascular endothelial growth factor (VEGF) is a key angiogenic factor, and vascular change is one of the features of airway remodeling. We examined the effect of TGF-β1 on VEGF production by fibroblasts from mice lacking expression of Smad2 or Smad3 as well as human lung fibroblasts treated with or without Smad2 or Smad3 siRNA. TGF-β1 stimulated VEGF production by fibroblasts from Smad2 deficient animals and wildtype animals. In contrast, TGF-β1 did not affect VEGF production by fibroblasts from Samd3 deficient mice. Similarly, TGF-β1 failed to stimulate VEGF production by HFL-1 cells treated with Samd3 siRNA but significantly increased VEGF production by the cells treated with Smad2 siRNA. These result suggest that TGF-β1 stimulation of VEGF production by fibroblasts is regulated by Smad3 but not by Smad2 signaling.

Original languageEnglish (US)
Pages (from-to)393-398
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume327
Issue number2
DOIs
StatePublished - Feb 11 2005

Fingerprint

Transforming Growth Factors
Fibroblasts
Vascular Endothelial Growth Factor A
Lung
Small Interfering RNA
Animals
Physiological Phenomena
Airway Remodeling
Angiogenesis Inducing Agents
Pathologic Processes
Blood Vessels

Keywords

  • Smad2
  • Smad3
  • Small interference RNA
  • Transforming growth factor-β1
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Smad3 mediates TGF-β1 induction of VEGF production in lung fibroblasts. / Kobayashi, Tetsu; Liu, Xiang-de; Wen, Fu Qiang; Fang, Qiuhong; Abe, Shinji; Wang, Xiang Qi; Hashimoto, Mitsuyoshi; Shen, Lei; Kawasaki, Shin; Kim, Hui Jung; Kohyama, Tadashi; Rennard, Stephen I.

In: Biochemical and Biophysical Research Communications, Vol. 327, No. 2, 11.02.2005, p. 393-398.

Research output: Contribution to journalArticle

Kobayashi, T, Liu, X, Wen, FQ, Fang, Q, Abe, S, Wang, XQ, Hashimoto, M, Shen, L, Kawasaki, S, Kim, HJ, Kohyama, T & Rennard, SI 2005, 'Smad3 mediates TGF-β1 induction of VEGF production in lung fibroblasts', Biochemical and Biophysical Research Communications, vol. 327, no. 2, pp. 393-398. https://doi.org/10.1016/j.bbrc.2004.12.032
Kobayashi, Tetsu ; Liu, Xiang-de ; Wen, Fu Qiang ; Fang, Qiuhong ; Abe, Shinji ; Wang, Xiang Qi ; Hashimoto, Mitsuyoshi ; Shen, Lei ; Kawasaki, Shin ; Kim, Hui Jung ; Kohyama, Tadashi ; Rennard, Stephen I. / Smad3 mediates TGF-β1 induction of VEGF production in lung fibroblasts. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 327, No. 2. pp. 393-398.
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