Smad3 mediates TGF-β1-induced collagen gel contraction by human lung fibroblasts

Tetsu Kobayashi, Xiang-de Liu, Fu Qiang Wen, Tadashi Kohyama, Lei Shen, Xing Qi Wang, Mitsuyoshi Hashimoto, Lijun Mao, Shinsaku Togo, Shin Kawasaki, Hisatoshi Sugiura, Koichiro Kamio, Stephen I. Rennard

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Transforming growth factor-β1 (TGF-β1) is a key mediator in tissue repair and fibrosis. Using small interference RNA (siRNA), the role of Smad2 and Smad3 in TGF-β stimulation of human lung fibroblast contraction of collagenous matrix and induction of α-SMA and the role of α-SMA in contraction were assessed. HFL-1 cells were transfected with Smad2, Smad3 or control-siRNA, and cultured in floating Type I collagen gels ±-TGF-β1. TGF-β1 augmented gel contraction in Smad2-siRNA- and control-siRNA-treated cells, but had no effect in Smad3-siRNA-treated cells. Similarly, TGF-β1 upregulated α-SMA in Smad2-siRNA- and control-siRNA-treated cells, but had no effect on Smad3-siRNA-treated cells. α-SMA-siRNA-treated cells did not contact the collagen gels with or without TGF-β1, suggesting α-SMA is required for gel contraction. Thus, Smad3 mediates TGF-β1-induced contraction and α-SMA induction in human lung fibroblasts. Smad3, therefore, could be a target for blocking contraction of human fibrotic tissue induced by TGF-β1.

Original languageEnglish (US)
Pages (from-to)290-295
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume339
Issue number1
DOIs
StatePublished - Jan 6 2006

Fingerprint

Transforming Growth Factors
Fibroblasts
RNA Interference
Collagen
Gels
RNA
Lung
Cells
Tissue
Collagen Type I
Fibrosis
Repair

Keywords

  • Fibroblast
  • Smad3
  • Small interference RNA
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Smad3 mediates TGF-β1-induced collagen gel contraction by human lung fibroblasts. / Kobayashi, Tetsu; Liu, Xiang-de; Wen, Fu Qiang; Kohyama, Tadashi; Shen, Lei; Wang, Xing Qi; Hashimoto, Mitsuyoshi; Mao, Lijun; Togo, Shinsaku; Kawasaki, Shin; Sugiura, Hisatoshi; Kamio, Koichiro; Rennard, Stephen I.

In: Biochemical and Biophysical Research Communications, Vol. 339, No. 1, 06.01.2006, p. 290-295.

Research output: Contribution to journalArticle

Kobayashi, T, Liu, X, Wen, FQ, Kohyama, T, Shen, L, Wang, XQ, Hashimoto, M, Mao, L, Togo, S, Kawasaki, S, Sugiura, H, Kamio, K & Rennard, SI 2006, 'Smad3 mediates TGF-β1-induced collagen gel contraction by human lung fibroblasts', Biochemical and Biophysical Research Communications, vol. 339, no. 1, pp. 290-295. https://doi.org/10.1016/j.bbrc.2005.10.209
Kobayashi, Tetsu ; Liu, Xiang-de ; Wen, Fu Qiang ; Kohyama, Tadashi ; Shen, Lei ; Wang, Xing Qi ; Hashimoto, Mitsuyoshi ; Mao, Lijun ; Togo, Shinsaku ; Kawasaki, Shin ; Sugiura, Hisatoshi ; Kamio, Koichiro ; Rennard, Stephen I. / Smad3 mediates TGF-β1-induced collagen gel contraction by human lung fibroblasts. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 339, No. 1. pp. 290-295.
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