SMA1, a homolog of the splicing factor Prp28, has a multifaceted role in miRNA biogenesis in Arabidopsis

Shengjun Li, Ran Xu, Aixia Li, Kan Liu, Liqing Gu, Mu Li, Hairui Zhang, Yueying Zhang, Shangshang Zhuang, Quanhui Wang, Gang Gao, Na Li, Chi Zhang, Yunhai Li, Bin Yu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress gene expression. In plants, the RNase III enzyme Dicer-like (DCL1) processes primary miRNAs (pri-miRNAs) into miRNAs. Here, we show that SMALL1 (SMA1), a homolog of the DEAD-box pre-mRNA splicing factor Prp28, plays essential roles in miRNA biogenesis in Arabidopsis. A hypomorphic sma1-1 mutation causes growth defects and reduces miRNA accumulation correlated with increased target transcript levels. SMA1 interacts with the DCL1 complex and positively influences pri-miRNA processing. Moreover, SMA1 binds the promoter region of genes encoding pri-miRNAs (MIRs) and is required for MIR transcription. Furthermore, SMA1 also enhances the abundance of the DCL1 protein levels through promoting the splicing of the DCL1 pre-mRNAs. Collectively, our data provide new insights into the function of SMA1/Prp28 in regulating miRNA abundance in plants.

Original languageEnglish (US)
Pages (from-to)9148-9159
Number of pages12
JournalNucleic acids research
Volume46
Issue number17
DOIs
StatePublished - Jan 1 2018

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MicroRNAs
Arabidopsis
Ribonuclease III
RNA Precursors
RNA Splicing Factors
Small Untranslated RNA
Genetic Promoter Regions
Gene Expression
Mutation
Growth
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

SMA1, a homolog of the splicing factor Prp28, has a multifaceted role in miRNA biogenesis in Arabidopsis. / Li, Shengjun; Xu, Ran; Li, Aixia; Liu, Kan; Gu, Liqing; Li, Mu; Zhang, Hairui; Zhang, Yueying; Zhuang, Shangshang; Wang, Quanhui; Gao, Gang; Li, Na; Zhang, Chi; Li, Yunhai; Yu, Bin.

In: Nucleic acids research, Vol. 46, No. 17, 01.01.2018, p. 9148-9159.

Research output: Contribution to journalArticle

Li, S, Xu, R, Li, A, Liu, K, Gu, L, Li, M, Zhang, H, Zhang, Y, Zhuang, S, Wang, Q, Gao, G, Li, N, Zhang, C, Li, Y & Yu, B 2018, 'SMA1, a homolog of the splicing factor Prp28, has a multifaceted role in miRNA biogenesis in Arabidopsis', Nucleic acids research, vol. 46, no. 17, pp. 9148-9159. https://doi.org/10.1093/nar/gky591
Li, Shengjun ; Xu, Ran ; Li, Aixia ; Liu, Kan ; Gu, Liqing ; Li, Mu ; Zhang, Hairui ; Zhang, Yueying ; Zhuang, Shangshang ; Wang, Quanhui ; Gao, Gang ; Li, Na ; Zhang, Chi ; Li, Yunhai ; Yu, Bin. / SMA1, a homolog of the splicing factor Prp28, has a multifaceted role in miRNA biogenesis in Arabidopsis. In: Nucleic acids research. 2018 ; Vol. 46, No. 17. pp. 9148-9159.
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abstract = "MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress gene expression. In plants, the RNase III enzyme Dicer-like (DCL1) processes primary miRNAs (pri-miRNAs) into miRNAs. Here, we show that SMALL1 (SMA1), a homolog of the DEAD-box pre-mRNA splicing factor Prp28, plays essential roles in miRNA biogenesis in Arabidopsis. A hypomorphic sma1-1 mutation causes growth defects and reduces miRNA accumulation correlated with increased target transcript levels. SMA1 interacts with the DCL1 complex and positively influences pri-miRNA processing. Moreover, SMA1 binds the promoter region of genes encoding pri-miRNAs (MIRs) and is required for MIR transcription. Furthermore, SMA1 also enhances the abundance of the DCL1 protein levels through promoting the splicing of the DCL1 pre-mRNAs. Collectively, our data provide new insights into the function of SMA1/Prp28 in regulating miRNA abundance in plants.",
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AU - Xu, Ran

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AU - Gu, Liqing

AU - Li, Mu

AU - Zhang, Hairui

AU - Zhang, Yueying

AU - Zhuang, Shangshang

AU - Wang, Quanhui

AU - Gao, Gang

AU - Li, Na

AU - Zhang, Chi

AU - Li, Yunhai

AU - Yu, Bin

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N2 - MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress gene expression. In plants, the RNase III enzyme Dicer-like (DCL1) processes primary miRNAs (pri-miRNAs) into miRNAs. Here, we show that SMALL1 (SMA1), a homolog of the DEAD-box pre-mRNA splicing factor Prp28, plays essential roles in miRNA biogenesis in Arabidopsis. A hypomorphic sma1-1 mutation causes growth defects and reduces miRNA accumulation correlated with increased target transcript levels. SMA1 interacts with the DCL1 complex and positively influences pri-miRNA processing. Moreover, SMA1 binds the promoter region of genes encoding pri-miRNAs (MIRs) and is required for MIR transcription. Furthermore, SMA1 also enhances the abundance of the DCL1 protein levels through promoting the splicing of the DCL1 pre-mRNAs. Collectively, our data provide new insights into the function of SMA1/Prp28 in regulating miRNA abundance in plants.

AB - MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress gene expression. In plants, the RNase III enzyme Dicer-like (DCL1) processes primary miRNAs (pri-miRNAs) into miRNAs. Here, we show that SMALL1 (SMA1), a homolog of the DEAD-box pre-mRNA splicing factor Prp28, plays essential roles in miRNA biogenesis in Arabidopsis. A hypomorphic sma1-1 mutation causes growth defects and reduces miRNA accumulation correlated with increased target transcript levels. SMA1 interacts with the DCL1 complex and positively influences pri-miRNA processing. Moreover, SMA1 binds the promoter region of genes encoding pri-miRNAs (MIRs) and is required for MIR transcription. Furthermore, SMA1 also enhances the abundance of the DCL1 protein levels through promoting the splicing of the DCL1 pre-mRNAs. Collectively, our data provide new insights into the function of SMA1/Prp28 in regulating miRNA abundance in plants.

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