Slow virus-macrophage interactions. Characterization of a transformed cell line of sheep alveolar macrophages that express a marker for susceptibility to ovine-caprine lentivirus infections

Howard Eliot Gendelman, O. Narayan, S. Kennedy-Stoskopf, J. E. Clements, G. H. Pezeshkpour

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Visna-maedi of sheep and arthritis encephalitis of goats are slowly progressive diseases caused by serologically related lentiviruses. Lesions are inflammatory and can occur at one or many sites including the central nervous system, lungs, joints, and mammary glands. The viruses replicate in macrophages, and in the animal large numers of infected macrophages can be obtained from inflamed tissues. To study virus-macrophage interactions we transformed sheep alveolar macrophages, which are natural virus target cells, with simian virus 40 and produced a macrophage cell line. The transformed cells grew into density-dependent monolayers and were subcultured after trypsin dissociation. They maintained histochemical and physiologic properties of macrophages as well as the ability to support replication of the lentiviruses. Rabbit antisera to these cells reacted with blood monocytes and only selected populations of tissue macrophages, including those in lung, synovium, mammary gland, and spleen. Microglia, Kupffer cells, and connective tissue histiocytes were not recognized by the sera. Since the tissues in which virus localizes in infected animals are the same as those recognized by the sera, the antimacrophage serum may provide an immunologic marker for virus-susceptible macrophages in the animal.

Original languageEnglish (US)
Pages (from-to)547-555
Number of pages9
JournalLaboratory Investigation
Volume51
Issue number5
StatePublished - Dec 1 1984

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Ovine-Caprine Lentiviruses
Lentivirus Infections
Transformed Cell Line
Alveolar Macrophages
Sheep
Macrophages
Viruses
Lentivirus
Human Mammary Glands
Progressive Interstitial Pneumonia of Sheep
Visna
Lung
Histiocytes
Kupffer Cells
Simian virus 40
Synovial Membrane
Microglia
Encephalitis
Serum
Goats

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Slow virus-macrophage interactions. Characterization of a transformed cell line of sheep alveolar macrophages that express a marker for susceptibility to ovine-caprine lentivirus infections. / Gendelman, Howard Eliot; Narayan, O.; Kennedy-Stoskopf, S.; Clements, J. E.; Pezeshkpour, G. H.

In: Laboratory Investigation, Vol. 51, No. 5, 01.12.1984, p. 547-555.

Research output: Contribution to journalArticle

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