Skeletal muscle cold shock and heat shock protein mRNA response to aerobic exercise in different environmental temperatures

Rebecca L. Cuthbert, Robert J. Shute, Dustin R. Slivka

Research output: Contribution to journalArticle

Abstract

The response of cold shock proteins to exercise and environmental temperature in human skeletal muscle is not known. The purpose of this study was to determine the early mRNA response of human stress proteins to endurance exercise and environmental temperatures. Seven recreationally trained males cycled for 1 hour at 60% VO2peak in 7°C, 20°C, and 33°C with biopsies taken pre- and 3 hours post-exercise. Gene expression for heat shock and cold shock proteins were analyzed using qRT-PCR on muscle biopsy samples from the vastus lateralis. RBM3 mRNA was reduced 1.43 ± 0.10 fold (p = 0.006) while there was a trend for CIRP to decrease1.27 ± 0.14 fold (p = 0.059) from pre- to 3 h post-exercise. CIRP and RBM3 mRNA were not different between temperatures (p = 0.273 and p = 0.686, respectively). HSP70 mRNA was 2.27 ± 0.23 fold higher 3 h post-exercise when compared to pre-exercise (p = 0.002) but was not significantly different between temperatures (p = 0.103). HSP27, HSP90, and HSF1 mRNA did not change from pre- to post-exercise (p = 0.052, p = 0.324, p = 0.795) and were not different between temperatures (p = 0.247, p = 0.134, p = 0.808). These data indicate that exposure to mild heat and cold during aerobic exercise have limited effect on the skeletal muscle mRNA expression of heat shock and cold shock proteins. However, skeletal muscle mRNA of cold shock proteins decrease, while HSP70 mRNA increases in response to a low to moderate intensity aerobic exercise bout.

Original languageEnglish (US)
Pages (from-to)77-84
Number of pages8
JournalTemperature
Volume6
Issue number1
DOIs
StatePublished - Jan 2 2019

Fingerprint

Heat-Shock Proteins
Shock
Skeletal Muscle
Cold Shock Proteins and Peptides
Exercise
Messenger RNA
Temperature
Hot Temperature
Biopsy
Quadriceps Muscle
Gene Expression
Muscles
Polymerase Chain Reaction

Keywords

  • CIRP
  • Human
  • RBM3
  • endurance
  • gene expression
  • physical activity

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Skeletal muscle cold shock and heat shock protein mRNA response to aerobic exercise in different environmental temperatures. / Cuthbert, Rebecca L.; Shute, Robert J.; Slivka, Dustin R.

In: Temperature, Vol. 6, No. 1, 02.01.2019, p. 77-84.

Research output: Contribution to journalArticle

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