Size-dependent fluorescence of bioaerosols

Mathematical model using fluorescing and absorbing molecules in bacteria

Steven C. Hill, Chatt C. Williamson, David C. Doughty, Yong Le Pan, Joshua L Santarpia, Hanna H. Hill

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are amino acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266nm. The emission range, 300 to 370nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (C F ) is calculated and compared with one set of published measured values. We investigate power law (Ad y ) approximations to C F , where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle[U+05F3]s fluorescence efficiency (Q F =C F /geometric-cross-section) can be written for homogeneous particles as Q abs R F , where Q abs is the absorption efficiency, and R F , the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When Q F is plotted vs. m i d or m i (m r -1)d, where m=m r +im i is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.

Original languageEnglish (US)
Pages (from-to)54-70
Number of pages17
JournalJournal of Quantitative Spectroscopy and Radiative Transfer
Volume157
DOIs
StatePublished - May 1 2015
Externally publishedYes

Fingerprint

bacteria
mathematical models
Bacteria
Fluorescence
Mathematical models
fluorescence
Molecules
molecules
Water
Coenzymes
Ovalbumin
Bacilli
Tryptophan
Nucleic Acids
coenzymes
Refractive index
Bacillus
tryptophan
cross sections
nucleic acids

Keywords

  • Aerosol characterization
  • Bioaerosols
  • Fluorescence
  • Light scattering

ASJC Scopus subject areas

  • Radiation
  • Atomic and Molecular Physics, and Optics
  • Spectroscopy

Cite this

Size-dependent fluorescence of bioaerosols : Mathematical model using fluorescing and absorbing molecules in bacteria. / Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong Le; Santarpia, Joshua L; Hill, Hanna H.

In: Journal of Quantitative Spectroscopy and Radiative Transfer, Vol. 157, 01.05.2015, p. 54-70.

Research output: Contribution to journalArticle

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