Six strains of human immunodeficiency virus type 1 isolated in Japan and their molecular phylogeny

Nobuaki Shimizu, Yashuhiro Takeuchi, Takuji Naruse, Minoru Inagaki, Etsuko Moriyama, Takashi Gojobori, Hiroo Hoshino

Research output: Contribution to journalArticle

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Abstract

Five strains of human immunodeficiency virus type 1 (HIV-1) were isolated from five Japanese hemophilia patients. Two isolates, HIV1[GUN-1] and HIV-1[GUN-2], were from brother patients with hemophilia B and the other three isolates, HIV-I[GUN-3], HIV-1[GUN-4], and HIV1[GUN-5], were from hemophilia A patients. Another HIV-1 strain, HIV-1[GUN-6], was isolated from a Canadian male homosexual with AIDS. The restriction endonuclease cleavage maps of the proviral genomes of these six HIV-1 strains revealed that they were apparently different from each other. The phylogenetic trees constructed using restriction maps and nucleotide sequences were quite similar, indicating that phylogenetic analyses of Japanese HIV-1 isolates can be done using restriction maps of the proviruses. Phylogenetic analyses showed that they were more closely related to HIV-1s which had been reported to be isolated from homosexual patients in the United States than those isolated from African patients. In particular, GUN-1 and GUN-2 isolates were on the branch of a San Francisco isolate, ARV2, while GUN-5 and GUN-6 isolates were on the branch of HTLV-III B -related isolates.

Original languageEnglish (US)
Pages (from-to)329-336
Number of pages8
JournalJournal of Molecular Evolution
Volume35
Issue number4
DOIs
StatePublished - Jan 1 1992

Fingerprint

Phylogeny
human immunodeficiency virus
Human immunodeficiency virus 1
HIV-1
Japan
phylogeny
hemophilia
Hemophilia A
phylogenetics
proviruses
HIV
Hemophilia B
Proviruses
restriction endonucleases
San Francisco
DNA Restriction Enzymes
acquired immune deficiency syndrome
Siblings
cleavage
Acquired Immunodeficiency Syndrome

Keywords

  • AIDS
  • HIV-1 isolate
  • Japanese hemophiliac
  • Phylogenetic tree
  • Restriction map

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Six strains of human immunodeficiency virus type 1 isolated in Japan and their molecular phylogeny. / Shimizu, Nobuaki; Takeuchi, Yashuhiro; Naruse, Takuji; Inagaki, Minoru; Moriyama, Etsuko; Gojobori, Takashi; Hoshino, Hiroo.

In: Journal of Molecular Evolution, Vol. 35, No. 4, 01.01.1992, p. 329-336.

Research output: Contribution to journalArticle

Shimizu, Nobuaki ; Takeuchi, Yashuhiro ; Naruse, Takuji ; Inagaki, Minoru ; Moriyama, Etsuko ; Gojobori, Takashi ; Hoshino, Hiroo. / Six strains of human immunodeficiency virus type 1 isolated in Japan and their molecular phylogeny. In: Journal of Molecular Evolution. 1992 ; Vol. 35, No. 4. pp. 329-336.
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