siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis

Bin Yu, Liu Bi, Jixian Zhai, Manu Agarwal, Shengben Li, Qingfa Wu, Shou Wei Ding, Blake C. Meyers, Hervé Vaucheret, Xuemei Chen

Research output: Contribution to journalArticle

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Abstract

Plant microRNAs (miRNAs) and small interfering RNAs (siRNAs) bear a 2'-O-methyl group on the 3'-terminal nucleotide. This methyl group is post-synthetically added by the methyltransferase protein HEN1 and protects small RNAs from enzymatic activities that target the 3'-OH. A mutagenesis screen for suppressors of the partial loss-of-function hen1-2 allele in Arabidopsis identified second-site mutations that restore miRNA methylation. These mutations affect two subunits of the DNA-dependent RNA polymerase IV (Pol IV), which is essential for the biogenesis of 24 nt endogenous siRNAs. A mutation in RNA-dependent RNA polymerase 2, another essential gene for the biogenesis of endogenous 24-nt siRNAs, also rescued the defects in miRNA methylation of hen1-2, revealing a previously unsuspected, negative influence of siRNAs on HEN1-mediated miRNA methylation. In addition, our findings imply the existence of a negative modifier of HEN1 activity in the Columbia genetic background.

Original languageEnglish (US)
Article numbergkq348
Pages (from-to)5844-5850
Number of pages7
JournalNucleic acids research
Volume38
Issue number17
DOIs
StatePublished - May 6 2010

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MicroRNAs
Arabidopsis
Methylation
Small Interfering RNA
Mutation
Protein Methyltransferases
RNA Replicase
Essential Genes
DNA-Directed RNA Polymerases
Mutagenesis
Nucleotides
Alleles
RNA

ASJC Scopus subject areas

  • Genetics

Cite this

Yu, B., Bi, L., Zhai, J., Agarwal, M., Li, S., Wu, Q., ... Chen, X. (2010). siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis. Nucleic acids research, 38(17), 5844-5850. [gkq348]. https://doi.org/10.1093/nar/gkq348

siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis. / Yu, Bin; Bi, Liu; Zhai, Jixian; Agarwal, Manu; Li, Shengben; Wu, Qingfa; Ding, Shou Wei; Meyers, Blake C.; Vaucheret, Hervé; Chen, Xuemei.

In: Nucleic acids research, Vol. 38, No. 17, gkq348, 06.05.2010, p. 5844-5850.

Research output: Contribution to journalArticle

Yu, B, Bi, L, Zhai, J, Agarwal, M, Li, S, Wu, Q, Ding, SW, Meyers, BC, Vaucheret, H & Chen, X 2010, 'siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis', Nucleic acids research, vol. 38, no. 17, gkq348, pp. 5844-5850. https://doi.org/10.1093/nar/gkq348
Yu B, Bi L, Zhai J, Agarwal M, Li S, Wu Q et al. siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis. Nucleic acids research. 2010 May 6;38(17):5844-5850. gkq348. https://doi.org/10.1093/nar/gkq348
Yu, Bin ; Bi, Liu ; Zhai, Jixian ; Agarwal, Manu ; Li, Shengben ; Wu, Qingfa ; Ding, Shou Wei ; Meyers, Blake C. ; Vaucheret, Hervé ; Chen, Xuemei. / siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis. In: Nucleic acids research. 2010 ; Vol. 38, No. 17. pp. 5844-5850.
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