Single transmembrane domain insulin-like growth factor-II/mannose-6- phosphate receptor regulates central cholinergic function by activating a G-protein-sensitive, protein kinase C-dependent pathway

Cheryl Hawkes, Jack H. Jhamandas, Kim H. Harris, Wen Fu, Richard G MacDonald, Satyabrata Kar

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The insulin-like growth factor-II/mannose-6-phosphate (IGF-II/M6P) receptor is a single-pass transmembrane glycoprotein that plays an important role in the intracellular trafficking of lysosomal enzymes and endocytosis-mediated degradation of IGF-II. However, its role in signal transduction after IGF-II binding remains unclear. In the present study, we report that IGF-II/M6P receptor in the rat brain is coupled to a G-protein and that its activation by Leu27IGF-II, an analog that binds rather selectively to the IGF-II/M6P receptor, potentiates endogenous acetylcholine release from the rat hippocampal formation. This effect is mediated by a pertussis toxin (PTX)-sensitive GTP-binding protein and is dependent on protein kinase Cα(PKCα)-induced phosphorylation of downstream substrates, myristoylated alanine-rich C kinase substrate, and growth associated protein-43. Additionally, treatment with Leu27IGF-II causes a reduction in whole-cell currents and depolarization of cholinergic basal forebrain neurons. This effect, which is blocked by an antibody against the IGF-II/M6P receptor, is also sensitive to PTX and is mediated via activation of a PKC-dependent pathway. These results together revealed for the first time that the single transmembrane domain IGF-II/M6P receptor expressed in the brain is G-protein coupled and is involved in the regulation of central cholinergic function via the activation of specific intracellular signaling cascades.

Original languageEnglish (US)
Pages (from-to)585-596
Number of pages12
JournalJournal of Neuroscience
Volume26
Issue number2
DOIs
StatePublished - Jan 11 2006

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IGF Type 2 Receptor
Insulin-Like Growth Factor II
GTP-Binding Proteins
Protein Kinase C
Cholinergic Agents
Pertussis Toxin
GAP-43 Protein
Brain
Endocytosis
Acetylcholine
Signal Transduction
Hippocampus
Glycoproteins
Phosphorylation
Neurons
Antibodies
Enzymes

Keywords

  • Cholinergic neurons
  • G-protein-coupled receptor
  • Insulin-like growth factors
  • Intracellular signaling
  • Neurotransmitter release
  • Protein kinase C

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Single transmembrane domain insulin-like growth factor-II/mannose-6- phosphate receptor regulates central cholinergic function by activating a G-protein-sensitive, protein kinase C-dependent pathway. / Hawkes, Cheryl; Jhamandas, Jack H.; Harris, Kim H.; Fu, Wen; MacDonald, Richard G; Kar, Satyabrata.

In: Journal of Neuroscience, Vol. 26, No. 2, 11.01.2006, p. 585-596.

Research output: Contribution to journalArticle

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