Single strand confirmation polymorphism (SSCP) detection in exon I of the α-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)

Dinesh Kumar, Neelam Gupta, S. P.S. Ahlawat, R. Satyanarayana, Shyam Sunder, S. C. Gupta

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect α-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon 1 of the α-lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These α-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism (SNP) markers for association studies and marker assisted selection. Copyright by the Brazilian Society of Genetics.

Original languageEnglish (US)
Pages (from-to)287-289
Number of pages3
JournalGenetics and Molecular Biology
Volume29
Issue number2
DOIs
StatePublished - Jul 4 2006

Fingerprint

Lactalbumin
Goats
Exons
Milk
Genes
Polymerase Chain Reaction
Single Nucleotide Polymorphism
Population

Keywords

  • Alpha-lactalbumin
  • Genetic variabilty
  • Goat
  • Single-strand conformation polymorphism (SSCP)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Single strand confirmation polymorphism (SSCP) detection in exon I of the α-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus). / Kumar, Dinesh; Gupta, Neelam; Ahlawat, S. P.S.; Satyanarayana, R.; Sunder, Shyam; Gupta, S. C.

In: Genetics and Molecular Biology, Vol. 29, No. 2, 04.07.2006, p. 287-289.

Research output: Contribution to journalArticle

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