Single muscle fiber function with concurrent exercise or nutrition countermeasures during 60 days of bed rest in women

Scott Trappe, Andrew Creer, Dustin Slivka, Kiril Minchev, Todd Trappe

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

There is limited information on skeletal muscle properties in women with unloading and counter-measure programs to protect the unloading-induced atrophy. The current investigation tested the hypothesis that a concurrent aerobic and resistance exercise training program would preserve size and contractile function of slow- and fast-twitch muscle fibers. A secondary objective was to test the hypothesis that a leucine-enriched high-protein diet would partially attenuate single fiber characteristics. Vastus lateralis muscle biopsies were obtained before and on day 59 of bed rest from a control (BR; n = 8), nutrition (BRN; n = 8), or exercise (BRE; n = 8) group. Single muscle fibers were studied for diameter, peak force (Po), contractile velocity, and power. Those in the BR group had a decrease (P < 0.05) in myosin heavy chain (MHC) I diameter (-14%), Po (-35%), and power (-42%) and MHC IIa diameter (-16%) and Po (-31%; P < 0.06) and an increase (P < 0.05) in MHC hybrid fibers. Changes in size and function of MHC I (-19 to -44%) and IIa (-21% to -30%) fibers and MHC distribution in BRN individuals were similar to results in the BR group. In BRE conditions, MHC I and IIa size and contractile function were preserved during bed rest. These data show that the concurrent exercise program preserved the myocellular profile of the vastus lateralis muscle during 60-day bed rest. To combat muscle atrophy and function with long-term unloading, the exercise prescription program used in this study should be considered as a viable training program for the upper leg muscles, whereas the nutritional intervention used cannot be recommended as a countermeasure for skeletal muscle.

Original languageEnglish (US)
Pages (from-to)1242-1250
Number of pages9
JournalJournal of Applied Physiology
Volume103
Issue number4
DOIs
StatePublished - Oct 1 2007

Fingerprint

Bed Rest
Myosin Heavy Chains
Exercise
Muscles
Quadriceps Muscle
Skeletal Muscle
Slow-Twitch Muscle Fibers
Fast-Twitch Muscle Fibers
Education
Muscular Atrophy
Resistance Training
Leucine
Atrophy
Prescriptions
Leg
Diet
Biopsy
Proteins

Keywords

  • Contractile properties
  • Microgravity
  • Skeletal muscle
  • Spaceflight
  • WISE-2005 study

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Single muscle fiber function with concurrent exercise or nutrition countermeasures during 60 days of bed rest in women. / Trappe, Scott; Creer, Andrew; Slivka, Dustin; Minchev, Kiril; Trappe, Todd.

In: Journal of Applied Physiology, Vol. 103, No. 4, 01.10.2007, p. 1242-1250.

Research output: Contribution to journalArticle

Trappe, Scott ; Creer, Andrew ; Slivka, Dustin ; Minchev, Kiril ; Trappe, Todd. / Single muscle fiber function with concurrent exercise or nutrition countermeasures during 60 days of bed rest in women. In: Journal of Applied Physiology. 2007 ; Vol. 103, No. 4. pp. 1242-1250.
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