Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions

Jason A. Morrison, Rebecca McLennan, Lauren A. Wolfe, Madelaine M. Gogol, Samuel Meier, Mary C. McKinney, Jessica M. Teddy, Laura Holmes, Craig L Semerad, Andrew C. Box, Hua Li, Kathryn E. Hall, Anoja G. Perera, Paul M. Kulesa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Neural crest cells migrate throughout the embryo, but how cells move in a directed and collective manner has remained unclear. Here, we perform the first single-cell transcriptome analysis of cranial neural crest cell migration at three progressive stages in chick and identify and establish hierarchical relationships between cell position and time-specific transcriptional signatures. We determine a novel transcriptional signature of the most invasive neural crest Trailblazer cells that is consistent during migration and enriched for approximately 900 genes. Knockdown of several Trailblazer genes shows significant but modest changes to total distance migrated. However, in vivo expression analysis by RNAscope and immunohistochemistry reveals some salt and pepper patterns that include strong individual Trailblazer gene expression in cells within other subregions of the migratory stream. These data provide new insights into the molecular diversity and dynamics within a neural crest cell migratory stream that underlie complex directed and collective cell behaviors.

Original languageEnglish (US)
Article numbere28415
JournaleLife
Volume6
DOIs
StatePublished - Dec 4 2017

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Single-Cell Analysis
Neural Crest
Gene Expression Profiling
Genes
Gene expression
Salts
Molecular Dynamics Simulation
Cell Movement
Embryonic Structures
Immunohistochemistry
Gene Expression

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Morrison, J. A., McLennan, R., Wolfe, L. A., Gogol, M. M., Meier, S., McKinney, M. C., ... Kulesa, P. M. (2017). Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions. eLife, 6, [e28415]. https://doi.org/10.7554/eLife.28415

Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions. / Morrison, Jason A.; McLennan, Rebecca; Wolfe, Lauren A.; Gogol, Madelaine M.; Meier, Samuel; McKinney, Mary C.; Teddy, Jessica M.; Holmes, Laura; Semerad, Craig L; Box, Andrew C.; Li, Hua; Hall, Kathryn E.; Perera, Anoja G.; Kulesa, Paul M.

In: eLife, Vol. 6, e28415, 04.12.2017.

Research output: Contribution to journalArticle

Morrison, JA, McLennan, R, Wolfe, LA, Gogol, MM, Meier, S, McKinney, MC, Teddy, JM, Holmes, L, Semerad, CL, Box, AC, Li, H, Hall, KE, Perera, AG & Kulesa, PM 2017, 'Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions' eLife, vol. 6, e28415. https://doi.org/10.7554/eLife.28415
Morrison, Jason A. ; McLennan, Rebecca ; Wolfe, Lauren A. ; Gogol, Madelaine M. ; Meier, Samuel ; McKinney, Mary C. ; Teddy, Jessica M. ; Holmes, Laura ; Semerad, Craig L ; Box, Andrew C. ; Li, Hua ; Hall, Kathryn E. ; Perera, Anoja G. ; Kulesa, Paul M. / Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions. In: eLife. 2017 ; Vol. 6.
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