Single-cell protein profiling of wastewater enterobacterial communities predicts disinfection efficiency

Gomathinayagam Ponniah, Han Chen, Ronda Michielutti, Nancy Salonen, Paul Blum

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The efficiency of enterobacterial disinfection is dependent largely on enterobacterial community physiology. However, the relationship between enterobacterial community physiology and wastewater processing is unclear. The purpose of this study was to investigate this relationship. The influence of wastewater treatment processes on enterobacterial community physiology was examined at the single-cell level by using culture-independent methods. Intracellular concentrations of two conserved proteins, the growth-related protein Fis and the stationary-phase protein Dps, were analyzed by epifluoresence microscopy of uncultivated cells by using enterobacterial group-specific polyclonal fluorochrome-coupled antibodies. Enterobacterial single-cell community protein profiles were distinct for different types of biological treatment. The differences were not apparent when bulk methods of protein analysis were used. Trickling filter wastewater yielded Fis-enriched communities compared to the communities in submerged aeration basin wastewater. Community differences in Fis and Dps contents were used to predict disinfection efficiency. Disinfection of community samples by heat exposure combined with cultivation in selective media confirmed that enterobacterial communities exhibited significant differences in sensitivity to disinfection. These findings provide strategies that can be used to increase treatment plant performance, reduce the enterobacterial content in municipal wastewater, and minimize the release of disinfection by-products into receiving water.

Original languageEnglish (US)
Pages (from-to)4227-4235
Number of pages9
JournalApplied and environmental microbiology
Volume69
Issue number7
DOIs
StatePublished - Jul 1 2003

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single cell protein
Disinfection
disinfection
Waste Water
wastewater
protein
physiology
proteins
biological treatment
fluorescent dyes
selective media
cells
wastewater treatment
aeration
byproducts
antibody
Proteins
microscopy
single cell proteins
basins

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Single-cell protein profiling of wastewater enterobacterial communities predicts disinfection efficiency. / Ponniah, Gomathinayagam; Chen, Han; Michielutti, Ronda; Salonen, Nancy; Blum, Paul.

In: Applied and environmental microbiology, Vol. 69, No. 7, 01.07.2003, p. 4227-4235.

Research output: Contribution to journalArticle

Ponniah, Gomathinayagam ; Chen, Han ; Michielutti, Ronda ; Salonen, Nancy ; Blum, Paul. / Single-cell protein profiling of wastewater enterobacterial communities predicts disinfection efficiency. In: Applied and environmental microbiology. 2003 ; Vol. 69, No. 7. pp. 4227-4235.
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