Single-cell membrane drug delivery using porous pen nanodeposition

Yongliang Yang, Jing Yu, Amir Monemian Esfahani, Kristina Seiffert-Sinha, Ning Xi, Ilsoon Lee, Animesh A. Sinha, Liangliang Chen, Zhiyong Sun, Ruiguo Yang, Lixin Dong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Delivering molecules onto the plasma membrane of single cells is still a challenging task in profiling cell signaling pathways with single cell resolution. We demonstrated that a large quantity of molecules could be targeted and released onto the membrane of individual cells to trigger signaling responses. This is achieved by a porous pen nanodeposition (PPN) method, in which a multilayer porous structure, serving as a reservoir for a large amount of molecules, is formed on an atomic force microscope (AFM) tip using layer-by-layer assembly and post processing. To demonstrate its capability for single cell membrane drug delivery, PPN was employed to induce a calcium flux triggered by the binding of released antibodies to membrane antigens in an autoimmune skin disease model. This calcium signal propagates from the target cell to its neighbors in a matter of seconds, proving the theory of intercellular communication through cell-cell junctions. Collectively, these results demonstrated the effectiveness of PPN in membrane drug delivery for single cells; to the best of our knowledge, this is the first technique that can perform the targeted transport and delivery in single cell resolution, paving the way for probing complex signaling interactions in multicellular settings.

Original languageEnglish (US)
Pages (from-to)12704-12712
Number of pages9
JournalNanoscale
Volume10
Issue number26
DOIs
StatePublished - Jul 14 2018

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Cell membranes
Drug delivery
Membranes
Molecules
Calcium
Cell signaling
Antigens
Antibodies
Skin
Multilayers
Microscopes
Fluxes
Communication
Processing

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Yang, Y., Yu, J., Monemian Esfahani, A., Seiffert-Sinha, K., Xi, N., Lee, I., ... Dong, L. (2018). Single-cell membrane drug delivery using porous pen nanodeposition. Nanoscale, 10(26), 12704-12712. https://doi.org/10.1039/c8nr02600a

Single-cell membrane drug delivery using porous pen nanodeposition. / Yang, Yongliang; Yu, Jing; Monemian Esfahani, Amir; Seiffert-Sinha, Kristina; Xi, Ning; Lee, Ilsoon; Sinha, Animesh A.; Chen, Liangliang; Sun, Zhiyong; Yang, Ruiguo; Dong, Lixin.

In: Nanoscale, Vol. 10, No. 26, 14.07.2018, p. 12704-12712.

Research output: Contribution to journalArticle

Yang, Y, Yu, J, Monemian Esfahani, A, Seiffert-Sinha, K, Xi, N, Lee, I, Sinha, AA, Chen, L, Sun, Z, Yang, R & Dong, L 2018, 'Single-cell membrane drug delivery using porous pen nanodeposition', Nanoscale, vol. 10, no. 26, pp. 12704-12712. https://doi.org/10.1039/c8nr02600a
Yang Y, Yu J, Monemian Esfahani A, Seiffert-Sinha K, Xi N, Lee I et al. Single-cell membrane drug delivery using porous pen nanodeposition. Nanoscale. 2018 Jul 14;10(26):12704-12712. https://doi.org/10.1039/c8nr02600a
Yang, Yongliang ; Yu, Jing ; Monemian Esfahani, Amir ; Seiffert-Sinha, Kristina ; Xi, Ning ; Lee, Ilsoon ; Sinha, Animesh A. ; Chen, Liangliang ; Sun, Zhiyong ; Yang, Ruiguo ; Dong, Lixin. / Single-cell membrane drug delivery using porous pen nanodeposition. In: Nanoscale. 2018 ; Vol. 10, No. 26. pp. 12704-12712.
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