Single-amino-acid alterations in a highly conserved central region of vesicular stomatitis virus N protein differentially affect the viral nucleocapsid template functions

Debasis Nayak, Debasis Panda, Subash C. Das, Ming Luo, Asit K Pattnaik

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22 Citations (Scopus)

Abstract

The nucleocapsid protein (N) of vesicular stomatitis virus and other rhabdoviruses plays a central role in the assembly and template functions of the viral N-RNA complex. The crystal structure of the viral N-RNA complex suggests that the central region of the N protein interacts with the viral RNA. Sequence alignment of rhabdovirus N proteins revealed several highly conserved regions, one of which spanned residues 282 to 291 (GLSSKSPYSS) in the central region of the molecule. Alanine-scanning mutagenesis of this region suggested that replacement of the tyrosine residue at position 289 (Y289) with alanine resulted in an N-RNA template that is nonfunctional in viral genome replication and transcription. To establish the molecular basis of this defect, our further studies revealed that the Y289A mutant maintained its interaction with other N protein molecules but that its interactions with the P protein or with the viral RNA were defective. Replacement of Y289 with other aromatic, polar, or large amino acids indicated that the hydrophobic and aromatic nature of this position in the N protein is functionally important and that a larger aromatic residue is less favorable. Interestingly, we have observed that several single-amino-acid substitutions in this highly conserved region of the molecule rendered the nucleocapsid template nonfunctional in transcription without adversely affecting the replication functions. These results suggest that the structure of the N protein and the resulting N-RNA complex, in part, regulate the viral template functions in transcription and replication.

Original languageEnglish (US)
Pages (from-to)5525-5534
Number of pages10
JournalJournal of virology
Volume83
Issue number11
DOIs
StatePublished - Jun 1 2009

Fingerprint

Vesiculovirus
Nucleocapsid
nucleocapsid
Vesicular Stomatitis
Viral RNA
RNA
Viruses
Amino Acids
amino acids
Rhabdoviridae
aromatic compounds
Proteins
proteins
transcription (genetics)
Alanine
alanine
nucleocapsid proteins
Nucleocapsid Proteins
Sequence Alignment
Viral Genome

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Single-amino-acid alterations in a highly conserved central region of vesicular stomatitis virus N protein differentially affect the viral nucleocapsid template functions. / Nayak, Debasis; Panda, Debasis; Das, Subash C.; Luo, Ming; Pattnaik, Asit K.

In: Journal of virology, Vol. 83, No. 11, 01.06.2009, p. 5525-5534.

Research output: Contribution to journalArticle

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