Simultaneous measurement of glucose and glutamine in insect cell culture media by near infrared spectroscopy

Mark R. Riley, Martin Rhiel, Xiangji Zhou, Mark A. Arnold, David W. Murhammer

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

The purpose of this study was to develop noninvasive techniques to monitor the composition of cell culture media in insect cell bioreactors. Such a monitor could be used in conjunction with a fed-batch feeding scheme to ensure that cells are maintained in an optimal environment for growth and protein production. Glucose and glutamine concentrations in an insect cell culture bioreactor were determined off-line with near-infrared (NIR) absorption spectroscopy. Spectra were collected from 5000 to 4000 cm-1 with a 1.5-mm optical path length. Partial least squares (PLS) regression was applied to correlate the collected spectra with the concentration of the desired analytes. Under the culture conditions evaluated here, glucose and glutamine concentrations ranged from 38 to 55 mM and from 3 to 13 mM, respectively. Accurate measurements of glucose and glutamine in insect cell culture samples were possible over these entire ranges. The standard error of prediction (SEP) and mean percent error (MPE) for glutamine were 0.52 mM and 5.3%, respectively. Glucose could be measured with an SEP of 1.30 mM and MPE of 2.3%. These levels of error are quite low considering the changing complexity of the growth media due to the shifting levels of amino acids, carbohydrates, yeastolate, proteins, and cell debris. This study represents an important step in the development of noninvasive on-line monitoring devices for cell culture bioreactors.

Original languageEnglish (US)
Pages (from-to)11-15
Number of pages5
JournalBiotechnology and Bioengineering
Volume55
Issue number1
DOIs
StatePublished - Jul 5 1997

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Near infrared spectroscopy
Near-Infrared Spectroscopy
Glutamine
Cell culture
Glucose
Insects
Culture Media
Bioreactors
Cell Culture Techniques
Proteins
Growth
Least-Squares Analysis
Infrared absorption
Carbohydrates
Absorption spectroscopy
Debris
Amino acids
Infrared spectroscopy
Amino Acids
Equipment and Supplies

Keywords

  • Bioreactor monitoring
  • Insect cell culture
  • NIR spectroscopy

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Simultaneous measurement of glucose and glutamine in insect cell culture media by near infrared spectroscopy. / Riley, Mark R.; Rhiel, Martin; Zhou, Xiangji; Arnold, Mark A.; Murhammer, David W.

In: Biotechnology and Bioengineering, Vol. 55, No. 1, 05.07.1997, p. 11-15.

Research output: Contribution to journalArticle

Riley, Mark R. ; Rhiel, Martin ; Zhou, Xiangji ; Arnold, Mark A. ; Murhammer, David W. / Simultaneous measurement of glucose and glutamine in insect cell culture media by near infrared spectroscopy. In: Biotechnology and Bioengineering. 1997 ; Vol. 55, No. 1. pp. 11-15.
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